磷脂脂肪酸法分析野山参根区土壤微生物群落多样性  

Analysis of soil microbial diversity in wild ginseng with phospholipid fatty acid method

作　　者：张亚玉[1] 宋晓霞[1] 孙海[1] 汪景宽[2] 于树[2] 

机构地区：[1]中国农业科学院特产研究所,吉林吉林132109 [2]沈阳农业大学土壤与环境学院,沈阳110161

出　　处：《东北农业大学学报》2011年第2期92-96,共5页Journal of Northeast Agricultural University

基　　金：国家科技支撑计划(2007BAI38B01);吉林省科技厅计划项目(20090573)

摘　　要：应用磷脂脂肪酸法(PLFA)分析了野山参根区土壤及对照土壤微生物群落结构特征。结果表明,野山参生长的根区土壤与对照土壤相比,微生物总量发生了明显的变化,均有减少的趋势;野山参根区中代表有致病特征的真菌种类18??2w9,18??1w9c,18??1w9t的总量没有增加反而减少,这与以往的研究结果相反。应用主成分分析法从PLFAs中提出3个主成分,2个样品分别代表81.03%和85.44%的变异信息,主成分一(PC1)分别解释了36.54%和39.09%的变异,主成分二(PC2)分别解释了28.65%和29.91%的变异,主成分三(PC3)分别解释了15.83%和16.44%的变异,在测试的野山参根区土壤样品中,PC1得分均比对照低。而且从主成分得分图上可以很明显区分根区土壤与对照土壤。从微生物群落结构上揭示了野山参在一地健康生长的主要原因。The ester-linked phospholipid fatty acid（PLFA） method was used to evaluate the soil microbial community diversity in two wild ginseng root zone and corresponding control. The results showed that root zone campar with control,the total soil microbial biomass in root zone changed obviously and all depressed trend presented a tendency of decrease compared with that of control;the total represent fungi with the pathogenic efficacy 18：2w9,18：1w9c,18：1w9t in No.1 wild ginseng root zone was not increased and was decreased. The experiment result was different from all the results of the previous. Three principal components were screened out with the principal component analysis,represented 81.03% and 85.44% of the information of diversity. Among the three,PC1 explained 36.54% and 39.09% of the variation,PC2 explained 28.65% and 29.91%,PC3 explained 15.83% and 16.44%,which helped successfully distinguish root zone from control in the principal components scores charts. This was probably the reason why the wild ginseng could grow health long term.

关 键 词：土壤微生物 群落多样性 磷脂脂肪酸 主成分分析 野山参 

分 类 号：S151.94[农业科学—土壤学]