重组人Elafin对肝脏缺血-再灌注损伤的保护作用  

Effect of Recombinant Human Elafin on Hepatic Ischemia-Reperfusion Injury in Rat

作　　者：孙任任[1] 张赢予[1,2] 孙波[1] 张馨木[1] 

机构地区：[1]吉林大学药学院,吉林长春130021 [2]江苏大学附属人民医院心血管病研究所,江苏镇江212002

出　　处：《中国药业》2011年第5期6-7,共2页China Pharmaceuticals

摘　　要：目的探讨重组人Elafin在大鼠肝脏缺血-再灌注损伤中的保护作用。方法将50只大鼠随机均分为5组,即正常对照组、肝脏缺血-再灌注损伤模型组以及Elafin低、中、高剂量组。对Elafin低、中、高剂量组给予不同浓度的重组人Elafin进行治疗,然后取血液及肝脏标本,检测血清中丙氨酸氨基转移酶(ALT)、天门冬酸氨基转移酶(AST)及乳酸脱氢酶(LDH)水平,测定肝组织中髓过氧化物酶(MPO)和一氧化氮(NO)含量,并观察肝脏的病理组织学改变。结果缺血-再灌注后大鼠肝组织损害较重,血清ALT和AST以及LDH水平明显升高(P<0.001),肝组织中MPO和NO含量明显升高(P<0.001);若手术前给予大鼠一定浓度的重组人Elafin,可使ALT,AST,LDH,MPO及NO水平明显降低(P<0.05)。结论重组人Elafin对肝脏缺血-再灌注损伤有一定的保护作用。Objective To investigate the role of recombinant human Elafin in the pathogenesis of hepatic ischemia-reperfusion injury（HIRI）.Methods All rats were divided into 5 groups randomly,sham-operation group,model group,Elafin treatment groups（high,middle and low doses）,10 cases in each group.Elafin was given with high,middle and low doses in Elafin treatment groups.The rat model of HIRI was made.The contents of serum ALT,AST and LDH and liver MPO and NO were determined after the injection of Elafin.Meanwhile,the hepatic histopathological changes were observed.Results The liver showed severe hepatocytic damage after HIRI and the contents of serum ALT,AST and LDH and liver MPO and NO were significantly risen（P 0.001）.The injection of Elafin before operation could make the level of ALT,AST,LDH,MPO and NO significantly dropped（P 0.05）.Conclusion The recombinant human Elafin has a protective effect on the rat HIRI to some extent.

关 键 词：肝缺血-再灌注损伤 重组人Elafin 丙氨酸氨基转移酶 天门冬酸氨基转移酶 乳酸脱氢酶 髓过氧化物酶 一氧化氮 

分 类 号：R965[医药卫生—药理学] R977.3[医药卫生—药学]