硒对慢性氟中毒致雄性大鼠生殖毒性的拮抗作用  

Effect of combined exposure to fluoride and selenium on biochemical functions of the testis of male rats

作　　者：尉俊仁[1] 冯佩[1] 章子贵[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《浙江师范大学学报（自然科学版）》2011年第1期86-90,共5页Journal of Zhejiang Normal University:Natural Sciences

基　　金：国家自然科学基金资助项目(30871295)

摘　　要：为了探讨硒对氟致睾丸损伤的拮抗作用,以雄性SD大鼠为实验动物,在饮水中加氟化钠(50 mg/L)、亚硒酸钠(分别为0.375,0.750和1.500 mg/L),单纯喂养或硒氟两两组合喂养6个月,断头处死,用试剂盒测大鼠睾丸组织中乳酸脱氢酶(LDH)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)及三磷酸腺苷酶(ATPase)的活性.结果发现:1)大鼠体质量:氟加低硒组、氟加中硒组高于对照组,差异有统计学意义(P<0.05).2)LDH活性:染氟组低于对照组,中硒组、高硒组、氟加高硒组高于染氟组(P<0.05);AKP活性:染氟组低于对照组,而氟加中硒组高于对照组(P<0.05);ACP活性:染氟组高于对照组(P<0.05).3)Na+,K+-ATPase活性:高硒组及氟加高硒组较对照组降低;Ca2+-ATPase活性:中硒组和氟加高硒组高于对照组,差异均有统计学意义(P<0.05).研究表明硒可以对慢性氟中毒导致的雄性生殖毒性产生拮抗作用,不同酶对拮抗作用的敏感性不同.It was aimed to study the antagonism of selenium on male reproductive toxicity induced by excessive fluorine,the male SD rats deionized water supplemented with NaF or/and Na2SeO3 were fed for six months.The body weights,testical,epididymitis and its organic coefficient were measured and calculated;the levels of lactate dehydrogenase（LDH）,acid phosphatase（ACP）,alkaline phosphatase（AKP）,Na^＋K^＋-ATPase,Mg^2＋-ATPase,Ca^2＋-ATPase were examined.The results showed that： 1） Weigh of rats was significantly increased（P0.05） in group of fluorine plus low-dose selenium and fluorine plus mid-dose selenium compared to contrl.2） The level of LDH was significantly（P0.05） decreased in fluorine group compared to control and it was significantly（P0.05） increased in mid-dose selenium,high-dose selenium and fluorine plus high-dose selenium compared with fluorine group;The ACP level was significantly（P0.05） changed in fluorine group and fluorine plus mid-dose selenium compared to control respectively;similar significant（P0.05） changes were found in the level of AKP in fluorine group and control.3） Significant differences（P0.05） were observed in three groups in the level of Na＋K＋-ATPase;while the level of Ca2＋-ATPase in the mid-selenium group and fluorine plus high-dose selenium had significant（P0.05） differences from that of control.Fluoride resulted in reproductive toxicity in male rat,and appropriate selenium takin could antagonize the toxicity of flouride.

关 键 词：氟 硒 雄性生殖毒性 拮抗作用 

分 类 号：R994.6[医药卫生—毒理学]