人NIRF蛋白与HBV核心蛋白相互作用的初步研究  

Preliminary Study on the Interaction Between Human NIRF Protein and HBV Core Protein

作　　者：金芳敏[1,2] 钱冠华[1,2] 杨艳[1,2] 段昌柱[1,2] 

机构地区：[1]重庆医科大学细胞生物学与遗传学教研室,中国重庆400016 [2]重庆医科大学分子医学与肿瘤研究中心,中国重庆400016

出　　处：《生命科学研究》2011年第1期1-5,共5页Life Science Research

基　　金：国家自然科学基金资助项目(30872248);重庆市科技委员会资助项目(CSTC;2008BB5400);重庆市教育委员会资助项目(KJ080326)

摘　　要：人NIRF蛋白在细胞内是否能与HBV核心蛋白发生相互结合,目前尚不清楚.构建pcDNA3-HBC质粒,采用基因共转染和共表达技术,在真核细胞内进行NIRF与HBc的免疫荧光共定位实验,并通过免疫共沉淀实验进一步验证两种蛋白是否发生相互结合.结果表明在细胞内,NIRF能与HBc发生相互结合.The interaction between human NIRF protein and HBV core protein in vivo is not clear now.The plasmid of pcDNA3-HBC was contructed.It was performed with the technology of co-transfection and co-expression of genes.The immunofluorescence assay was used to find out the co-localization of NIRF and HBc.Co-immunoprecipitation was also used to further certify the experimenta1 result.The results showed that NIRF can bind to HBc in vivo.

关 键 词：NIRF HBC 免疫荧光 免疫共沉淀 

分 类 号：Q26[生物学—细胞生物学]