不同分化培养基对秋水仙碱处理大葱愈伤组织的影响  

Effects of Different Differentiation Media on Welsh Onion Callus

作　　者：高莉敏[1] 宗红[1] 陈运起[1] 高秀云 董飞[3] 

机构地区：[1]山东省农业科学院蔬菜研究所,山东济南250100 [2]成武县林业局,山东成武274200 [3]山东农业大学园艺科学与工程学院,山东泰安271018

出　　处：《山东农业科学》2011年第2期27-29,共3页Shandong Agricultural Sciences

基　　金：山东省农业科学院创新基金项目(2007ycx009);山东省良种产业化项目

摘　　要：采用正交设计,测定了不同分化培养基对秋水仙碱处理大葱愈伤组织的影响。结果表明,在不添加2,4-D的基础上,适当提高蔗糖浓度,可以减缓愈伤组织褐化现象的发生,提高芽分化率,以处理6(蔗糖40 g/L+琼脂15 g/L+6-BA 1.5 mg/L)加倍诱导率最高。试验得到了两种嵌合体植株,一种为同时具有三倍体和二倍体细胞的植株,另一种为同时具有四倍体和二倍体细胞的植株。The effects of different differentiation media on welsh onion callus treated with colchicin were determined by the orthogonal design experiment.The results showed that the appropriate increase of sucrose without 2,4-D could slow down the occurrence of browning phenomenon and increase the bud differentiation rate.The highest doubling induction rate could be obtained on the medium with 40 g/L sucrose,15 g/L agar and 1.5 mg/L 6-BA.Two kinds of chimera plants were acquired,which were the one with both triploid and diploid cells and the other one with both tetraploid and diploid cells.

关 键 词：大葱 愈伤组织 分化培养基 

分 类 号：S633.1[农业科学—蔬菜学] S335.3[农业科学—园艺学]