金黄色葡萄球菌攻击下小鼠泌乳期乳腺炎症病理机制分析  

Inflammation Pathology of Mouse Mammary Gland in Lactation Period Attacked by Staphylococcus aureus

作　　者：李丽[1,2] 魏春梅[2,3] 刘代成[1] 杨宏军[2] 何洪彬[2] 王长法[2] 高运东[2] 仲跻峰[2] 彭广能[3] 

机构地区：[1]山东师范大学生命科学学院,山东济南250014 [2]山东省农业科学院奶牛中心,山东济南250100 [3]四川农业大学动物医学院,四川雅安625014

出　　处：《山东农业科学》2011年第2期99-102,114,共5页Shandong Agricultural Sciences

基　　金：山东省中青年科学家基金项目(BS2009NY002);山东省农业重大应用技术创新课题(2009)资助

摘　　要：通过观察不同剂量(E1:10 CFU/μl;E2:20 CFU/μl;E3:100 CFU/μl;E4:200 CFU/μl;E5:2 000CFU/μl)金黄色葡萄球菌攻击下的小鼠泌乳期乳腺炎症病理变化,选择最佳剂量建立小鼠乳腺内感染模型。结果表明:试验组乳腺组织中分离的细菌数呈先升高后下降的趋势,E3达到最高。组织形态学观察结果:对照组腺泡结构完整;E1组和E2组腺泡内主要以浆液性渗出为主;E3组腺泡壁增厚,腺泡腔内嗜中性粒细胞(PMN)增多;随着剂量的增大E4和E5组腺泡壁破裂,甚至个别样品出现坏死灶。与对照组相比,E3组小鼠乳腺组织中TNF-α、IL-2和IL-6含量极显著升高(P(0.01)。100 CFU/μl(E3)剂量金黄色葡萄球菌攻击下的小鼠乳腺炎性反应与炎症的免疫病理过程一致,故选择此剂量作为小鼠乳腺内感染的病理模型的造模剂量。The pathological changes of mammary gland inflammation during lactation period of mice were observed under the attack by different doses of Staphylococcus aureus as 10 CFU/μl（E1）,20 CFU/μl（E2）,100 CFU/μl（E3）,200 CFU/μl（E4） and 2 000 CFU/μl（E5）,and the optimal dose was selected for the construction of infection model.The results were as follows.The number of bacteria isolated from mammary gland tissues of the experimental groups showed a downward trend after the first rise,and reached the highest at the E3 dose.The histopathologic examination of mammary gland revealed that the acinar structure of the control group were integrated;the E1 and E2 groups presented primarily serous effusion within the acinus;and the E3 group showed the number increase of polymorphonuclear neutrophils（PMN） and the thickening of the acinus wall.As the dose increased,the acinus wall of the E4 and E5 groups ruptured,and even necrotic foci appeared in individual samples.In comparison with the control group,the concentrations of TNF-,IL-2 and IL-6 of E3 group rose very significantly（P﹤0.01） in the mammary gland tissues.All the results demonstrated that the inflammation response under the attack of 100 CFU/μl（E3）Staphylococcus aureus was in accordance with the immunopathology,so this dose was chosen for the pathological modeling of the mice with mammary gland infection.

关 键 词：小鼠 金黄色葡萄球菌 乳腺内感染 炎症病理变化 

分 类 号：S858.917.26[农业科学—临床兽医学]