日本血吸虫感染小鼠肠系膜淋巴结T细胞亚群的变化  

The alteration of T cell subtype in mesenteric lymph node (MLN) in mice infected by Schistosome japonicum

作　　者：黄俊[1] 高志岩[1] 黎小妍[2] 谢红艳[3] 罗雪平[1] 

机构地区：[1]广州医学院病原生物学与免疫学教研室,广州510182 [2]中山大学附属第三医院药剂科,广州510630 [3]广州医学院机能学实验中心,广州510182

出　　处：《中国免疫学杂志》2011年第2期103-106,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金(No.30931353);广东省自然科学基金(No.093008)

摘　　要：目的:观察C57BL/6小鼠感染日本血吸虫(Schistosome japonicum,Sj)4～6周肠系膜淋巴结T细胞亚群的改变。方法:用Sj尾蚴腹贴法建立Sj感染的小鼠模型。4～6周后取肠系膜淋巴结做淋巴细胞计数,使用细胞内细胞因子染色的方法,利用流式细胞仪检测肠系膜淋巴结淋巴细胞中分泌不同细胞因子的T细胞亚群含量的变化。结果:Sj感染C57BL/6小鼠4～6周后,肠系膜淋巴结细胞数量明显增多;流式细胞仪检测发现肠系膜淋巴结中CD4+T细胞中分泌IFN-γ的Th1细胞增多1倍,分泌IL-4和IL-5的Th2细胞增多近20倍,Th1/Th2轴发生偏移;分泌IL-17的Th17细胞也增多近5倍;分泌IFN-γ的CD8+T细胞也增多1倍。结论:日本血吸虫感染C57BL/6小鼠4～6周肠系膜淋巴结细胞增多,并向Th2和Th17型细胞极化。Objective：To investigate the change of T cell subtypes in mesenteric lymph node（MLN） of C57BL/6 mice infected by Sj for 46 weeks.Methods：C57BL/6 mice were infected with Sj cercariea by applying abdomen walls.The numbers of MLN lymphocytes were counted after 4-6 weeks.Subtypes of T cells which secreted different cytokines were detected by flow cytometry.Results：The number of MLN lymphocytes in infected mice increased obviously.IFN-γ producing Th1 cells increased by 1 fold.IL-4 and IL-5 secreting Th2 cells increased by nearly 20 folds.The axis of Th1/Th2 shifted.In addition,IL-17 secreting Th17 cells were increased dramatically.Conclusion：The MLN of C57BL/6 mice infected with Sj has stored more lymphocytes,which polarizes to Th2 and Th17 phenotyoes.

关 键 词：日本血吸虫 肠系膜淋巴结 啦 THL7 

分 类 号：R392.9[医药卫生—免疫学]