TNF-α对肠上皮细胞PIgR、NF-κB亚单位表达影响  

The influence of TNF-α on the expression of PIgR and NF-κB subunit in intestinal epithelial cells

作　　者：刘冬妍[1] 苗佳宁[1] 丁鹏[1] 

机构地区：[1]中国医科大学附属盛京医院实验研究中心,沈阳110004

出　　处：《中国免疫学杂志》2011年第2期107-109,共3页Chinese Journal of Immunology

基　　金：国家自然科学基金(30871158);辽宁省教委课题资助项目(2008783)

摘　　要：目的:研究肿瘤坏死因子-α(TNF-α)对Caco-2细胞表达多聚免疫球蛋白受体(pIgR)和核因子-κB(NF-κB)亚单位RelA/RelB的影响。方法:采用Real-time PCR方法检测肠上皮细胞表达pIgR、RelA和RelB的变化。结果:与无TNF-α刺激比较,用不同浓度TNF-α刺激后pIgR、RelA和RelB基因的表达明显增加(P<0.01)。结论:TNF-α促进肠上皮细胞表达pIgR、NF-κB亚单位基因表达,TNF-α通过NF-κB通路激活pIgR基因的转录。Objective：To study the influence of tumor necrosis factor-α（TNF-α）on the expression of RIgR and RelA/RelB subunits of nuclear factor-kappa B（NF-κB）in Caco-2 cells.Methods：pIgR mRNA,RelA and RelB mRNA of Caco-2 cells were assayed by quantitative Real-time PCR.Results：The increase of expression of genes,RelA and RelB gene in Caco-2 cells was found by the stimulation of TNF-α treatment compared with that of untreatment（P〈0.01）.Conclusion：TNF-α stimulates the expression of RIgR genes,RelA/RelB genes in Caco-2 cells.TNF-α may activate transcription of the PIgR gene through the alternative NF-κB pathway.

关 键 词：多聚免疫球蛋白受体 核因子-ΚB 肿瘤坏死因子 CACO-2细胞 

分 类 号：R392.11[医药卫生—免疫学]