CD147蛋白和MMP-9在子宫腺肌病中的表达和意义  

Expression of CD147 protein and MMP-9 and the significance in patients with adenomyosis

作　　者：邹宁[1] 任云青[2] 李培莉[2] 薛丽萍 

机构地区：[1]山西医科大学,太原030001 [2]山西医科大学汾阳学院,汾阳032200 [3]山西省汾阳医院妇产科,汾阳032200

出　　处：《中国免疫学杂志》2011年第2期144-148,共5页Chinese Journal of Immunology

摘　　要：目的:研究CD147蛋白、MMP-9在子宫腺肌病患者组织中的表达及血清中可溶性CD147(sCD147)蛋白水平,探讨其在子宫腺肌病中的作用机制。方法:采用免疫组织化学SP法检测37例子宫腺肌病患者(子宫腺肌病组)子宫异位内膜、在位内膜及20例子宫肌瘤患者(对照组)子宫内膜组织中CD147蛋白和MMP-9的表达,并对其蛋白表达量进行相关性分析;采用ELISA双抗体夹心法检测37例子宫腺肌病患者血清sCD147蛋白的水平,20例子宫肌瘤患者和20例女性健康体检者设为对照。结果:子宫腺肌病组异位与在位子宫内膜组织腺上皮细胞、间质细胞均有CD147蛋白、MMP-9表达,且明显高于对照组(P<0.01),其中异位内膜CD147蛋白、MMP-9表达量高于在位内膜(P<0.01);子宫腺肌病组CD147蛋白与MMP-9的表达量成正相关,对照组两者则无相关性。子宫腺肌病组血清sCD147蛋白的水平高于子宫肌瘤组(P>0.05)和健康体检者组(P<0.05)。结论:子宫腺肌病患者异位与在位内膜组织中CD147蛋白和MMP-9高表达及血清sCD147蛋白高水平可能与子宫腺肌病发生发展有关,且CD147蛋白可能通过促进MMP-9合成与分泌而发挥其作用。Objective：To study the expression of CD147 and MMP-9 protein in patients with adenomyosis as well as their serum levels of soluble CD147 in order to investigate their roles in the pathogenesis of adenomyosis.Methods：Immunohistochemistry was performed to detect the expression of CD147 and MMP-9 protein in ectopic endometrium and eutopic endometrial tissues of 37 patients with adenomyosis（AM group） and endometrial tissues of 20 patients only with hysteromyoma（control group）.The correlation between the expressions of CD147 and MMP-9 protein was evaluated.ELISA was used to detect the level of soluble CD147 proteins in the peripheral blood of AM group（37 cases with adenomyosis）and control groups（20 cases with hysteromyoma and 20 healthy cases）.Results：The expression of CD147 and MMP-9 proteins in glandular and stromal cells in ectopic and eutopic endometrium of the patients with AM were significantly higher than in control group（P〈0.01）,and the expression of CD147 and MMP-9 protein was much higher in ectopic endometrium than in eutopic endometrium of the patients with AM.The expression of CD147 protein was positively correlated with MMP-9 in patient with AM.The serum levels of soluble CD147 was much higher in AM groups than in both control group with hysteromyoma（P〉0.05） and healthy group（P〈0.05）.Conclusion：The higher expression of CD147 protein and MMP-9 in ectopic and eutopic endometrium and serum levels of sCD147 may play an important role in the pathogenesis of AM,and CD147 protein plays its role probably through activating MMP-9 synthesis and secretion.

关 键 词：CD147 MMP-9 可溶性CD147 子宫腺肌病 

分 类 号：R711.71[医药卫生—妇产科学]