α-生育酚缓解链脲佐菌素诱导乳鼠胰岛细胞损伤的研究  

Effects of α-tocopherol on streptozotocin-induced apoptosis of neonatal rat pancreatic cells

作　　者：袁英[1] 张艳[1] 宋仕卿[1] 郭兵[1] 

机构地区：[1]重庆医药高等专科学校基础部,401331

出　　处：《重庆医学》2011年第7期676-677,682,I0004,共4页Chongqing medicine

摘　　要：目的探讨α-生育酚(α-T)对链脲佐菌素(STZ)诱导的胰岛细胞损伤的作用及其机制。方法原代培养大鼠胰岛细胞,电镜观察STZ处理前、后胰岛β细胞形态,检测STZ处理前、后胰岛细胞活性、胰岛细胞凋亡率以及Bcl-xl和Bax的变化。并进一步观察给予α-T预处理后上述指标的变化。结果 (1)随着α-T浓度增高,α-T干预组中反映胰岛细胞活性的吸光度(A)值呈剂量依赖性增加;(2)STZ组与空白对照组比较,胰岛细胞凋亡率增加(P<0.01),细胞Bcl-xl表达下降及Bax表达增强(P<0.01);(3)α-T干预组与STZ组比较,胰岛细胞凋亡率降低(P<0.01),细胞Bcl-xl表达增强及Bax表达下降(P<0.01)。结论α-T能够缓解STZ诱导大鼠胰岛细胞损伤,其机制可能与抑制胰岛细胞凋亡有关。Objective To investigate the effect of α-tocopherol on the streptozotocin-induced damage of rat islet cells.Methods Isolated islet cells from wistar rats of 1-3 d incubated in monolayer in vitro.β-cell morphology,islet cell viability,apoptotic rate of islet cells,expression of Bcl-xl and Bax after incubation with streptozotocin were measured,and the potential effects of α-tocopherol was investigated.Results（1）α-tocopherol increased the islet cell viability in a dose-dependent fashion.（2）Compared with the control group,apoptotic rate of pancreatic islet cells increased,Bcl-xl expression decreased and Bax gene expression increased when co-cultured with streptozotocin（P0.01）.（3）Compared with the STZ group,apoptotic rate of pancreatic islet cells decreased,Bcl-xl expression increased and Bax gene expression decreased in the α-tocopherol group（P0.01）.Conclusion α-tocopherol can effectively attenuate streptozotocin-induced damage of rat islet cells by means of regulating Bcl-xl and Bax gene expressions.

关 键 词：α生育酚 链脲菌素 胰岛素分泌细胞 bcl-X蛋白质 BCL-2相关X蛋白质 

分 类 号：R965[医药卫生—药理学]