银杏枝条营养贮藏蛋白质的免疫细胞化学鉴定  

Immunocytochemical Identification of Vegetative Storage Proteins in the Branch of Ginkgo biloba

作　　者：郭红彦[1,2] 彭方仁[2] 吴青霞[2] 白晋华[1] 

机构地区：[1]山西农业大学林学院,太谷030801 [2]南京林业大学森林资源与环境学院,南京210037

出　　处：《林业科学》2011年第1期68-72,共5页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(30872055);高校博士点基金(20070298008);山西农大科技创新基金项目(2008029);山西农业大学博士科研启动基金

摘　　要：依据银杏枝条中32和36ku蛋白质含量的明显季节变化,推测它们是银杏的营养贮藏蛋白质。采用间接免疫组织细胞化学定位技术,对这2种蛋白质进行细胞化学定位。32和36ku蛋白质具有一定的免疫相关性,它们分布在一些皮层薄壁细胞和韧皮部的韧皮薄壁细胞中,存在于这些细胞的小液泡里。研究结果揭示这2种蛋白质的组织分布式样,为这2种蛋白质是银杏的营养贮藏蛋白质提供有力证据。Both 32 ku and 36 ku proteins had been in speculated as the vegetative storage proteins the branches of Ginkgo biloba based on the obvious seasonal changes in their contents.The cytochemical localization of the two proteins was studied with the indirect immuohistochemistry localization technigue.The proteins with a certain immune correlation,were distributed in the vacuole of the parenchyma cells of cortex and the parenchyma cells of phloem.The results revealed tissue distribution pattern of the two kinds of protein,and in the meantime the results provided strong evidence to support the two kinds of protein as the vegetative storage proteins of G.biloba.

关 键 词：银杏 营养贮藏蛋白质 免疫细胞化学定位 

分 类 号：S718.43[农业科学—林学]