Growth and Physiological Features of Cyanobacterium Anabaena sp. Strain PCC 7120 in a Glucose-Mixotrophic Culture  

作　　者：喻国策 施定基 蔡昭铃 丛威 欧阳藩 

机构地区：[1]Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China [2]State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China [3]Research Center of Photosynthesis, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

出　　处：《Chinese Journal of Chemical Engineering》2011年第1期108-115,共8页中国化学工程学报（英文版）

基　　金：Supported by a grant from the State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences

摘　　要：Mixotrophic growth is one potential mode for mass culture of microalgae and cyanobacteria particularly suitable for the production of high value bioactive compounds and fine chemicals.The typical heterocystous cyanobacterium Anabaena sp.PCC 7120 was grown in the presence of exogenous glucose in light.Glucose improved the cell growth evidently,the maximal specific growth rate under mixotrophic condition（0.38 d 1）being 1.6-fold of that of photoautotrophic growth.Mixotrophy caused a variation in cellular pigment composition,increasing the content of chlorophyll a and decreasing the contents of carotenoid and phycobiliprotein relative to chlorophyll a.Fluorescence emission from photosystem II（PSII）relative to photosystem I was enhanced in mixotrophic cells,implying an increased energy distribution in PSII.Glucokinase（EC 2.7.1.2）activity was further induced in the presence of glucose.The mixotrophic culture was scaled up in a 15 L airlift photobioreactor equipped with an inner and an outer light source.A modified Monod model incorporating the specific growth rate and the average light intensity in the reactor was developed to describe cell growth appropriately.The understanding of mixotrophic growth and relevant physiological features of Anabaena sp.PCC 7120 would be meaningful for cultivation and exploitation of this important cyanobacterial strain.Mixotrophic 生长是为 microalgae 和对高价值 bioactive 混合物和好化学药品的生产特别地合适的 cyanobacteria 的集体文化的一个潜在的模式。典型 heterocystous cyanobacterium 淡水藻类的一种 sp。PCC 7120 在光面对外长的葡萄糖被种。葡萄糖显然改进了房间生长，在 mixotrophic 状况下面的最大的特定的生长率(0.38 d (1 ) photoautotrophic 生长的 1.6 褶层。Mixotrophy 在细胞的颜料作文引起了一个变化，增加叶绿素的内容 and 减少相对叶绿素的类胡萝卜素和 phycobiliprotein 的内容一。从相对 photosystem 的 photosystem II (PSII ) 的荧光排放我在 mixotrophic 房间被提高，在 PSII 暗示增加的精力分布。Glucokinase (EC 2.7.1.2 ) 活动进一步面对葡萄糖被导致。mixotrophic 文化在装备与的 15 L 空运 photobioreactor 被扩大规模一内部并且外部轻来源。合并特定的生长率和平均的光的一个修改 Monod 模型在反应堆的紧张被开发适当地描述细胞生长。mixotrophic 生长的理解和淡水藻类的一种 sp 的相关生理的特征。PCC 7120 将为这重要 cyanobacterial 紧张的耕作和利用是有意义的。

关 键 词：cyanobacteria Anabaena sp.PCC 7120 growth features mixotrophic culture PHOTOBIOREACTOR 

分 类 号：Q949.22[生物学—植物学] TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]