高粱DNA导入小麦“矮早8”引起后代变异的研究及SSR分析  

Study on Character Variation Induced by Broomcorn DNA Intrducing into the Wheat " Aizao8" and Assessment of Genetic Diversity of the Descendant by SSR Markers

作　　者：张希太 

机构地区：[1]邯郸市农业科学院生物技术中心,河北邯郸056001

出　　处：《安徽农学通报》2011年第5期41-43,57,共4页Anhui Agricultural Science Bulletin

基　　金：邯郸市科技发展计划

摘　　要：将高粱"抗5"DNA通过花粉管通道导入小麦品种"矮早8",其后代在株高、蜡质层、芒型、穗型、旗叶、成熟期等几个方面发生大量变异。通过对变异后代系统选择得到多个稳定的变异种质系;通过对"矮早8"、D8-1、D8-4、高粱"抗5"基因组DNA的SSR分子标记检测,引物xgwm6、xgwm148、xgwm295、xgwm328、xgwm257检测出了较丰富的多态性DNA片段,其中xgwm6、xgwm257检出了高粱"抗5"DNA特有而"矮早8"没有的特异带型,进一步说明了D8-1、D8-4是由高粱"抗5"DNA片段嵌入"矮早8"基因组DNA变异而来。There are many descendants had been mutated in plant height.waxiness .awn .Ear type.Flag leave .maturation by importing broomcorn DNA of ＂kang5＂ into the wheat ＂Aizao8＂ through the Pollen- tube Pathway~ We have own many unaltered germplasm line by Subsequent continuous selection in the variational descendants ; Detect the DNA of＂ aizao8＂ D8 - 1 .D8 -4.broomcorn＂ kang5＂ Using SSR markers, There are many differential fragments have been found by the Prime xgwm6 ,xgwm148 、 xgwm295, xgwm328, xgwm257, Especially xgwm6, xxgwm257, they have been found the differential fragments in the DNA of D8 - 1 and D8 - 4 that belong to the hroomcorn DNA of ＂kangS＂ only, It further attest that D8 - 1 and D8 -4 was come from ＂AizaoS＇＂ that was inlaid the fragments of broomcorn DNA of ＂kang5 ＂.

关 键 词：高粱DNA 花粉管通道 矮早8 后代变异 SSR分子标记 

分 类 号：S512.1[农业科学—作物学]