Rock-Ⅰ和MYPT-1在门静脉高压症脾静脉壁中的表达变化  

Expression of Rho - associated kinase I and myosine phosphatase targeting subunit 1 in the splenic venous wall of patients with portal hypertension

作　　者：王鹏[1] 王栋[1] 江雪[1] 鲁建国[1] 

机构地区：[1]第四军医大学唐都医院普通外科,西安710038

出　　处：《临床外科杂志》2011年第1期25-26,共2页Journal of Clinical Surgery

摘　　要：目的 研究Rho激酶（Rho-associated kinaseⅠ,Rock-Ⅰ）及其底物肌球蛋白磷酸酶肌球蛋白结合亚基1（myosine phosphatae targeting subunit 1,MYPT-1）在门静脉高压症脾静脉壁中的表达,初步探讨Rock信号转导通路在门静脉高压症时脾静脉病变中的作用.方法 实验组为门静脉高压症患者45例.住院期间患者接受断流或分流术脾切除.对照组为同期外伤性脾破裂行脾切除患者16例.采用免疫组化ABC法,Western Blot技术检测Rock-Ⅰ以及MYPT-1在脾静脉壁中的蛋白表达水平,并以MYPT-1的表达水平作为Rock-Ⅰ功能活化状态的指标.结果 Rock-Ⅰ和MYPT-1在门静脉高压症脾静脉壁中表达明显上调（P〈0.01）,Western Blot结果显示Rock-Ⅰ和MYPT-1的蛋白表达水平在实验组中明显升高（P〈0.01）,差异有统计学意义.实验组Rock-Ⅰ和MYPT-1的蛋白表达,二者呈显著正相关（r=0.858,P〈0.01）.结论 门静脉高压症患者脾静脉壁中明显存在Rock-Ⅰ表达上调与功能活化,提示Rho激酶信号转导通路在门静脉高压症脾静脉病变的发生机制中具有重要作用.Objective To detect the expression of Rho - associated kinase I （ Rock - I ） in the splenic venous walls of patients with portal hypertension and the phosphorylation level of its substrate myosine phosphatase targeting subunit 1 （ MYPT - 1 ）. Methods A total of 45 portal hypertension patients who underwent splenectomy in combination with portosystemic shunt and 16 patients who received splenectomy due to traumatic rupture were enrolled in the study. The expressions of Rock - I and MYPT - 1 in the splenic venous wall were detected by immunohistochemistry and western blotting, and the expression of MYPT- 1 was indicative of Rock - I activation. Result Immunohistochemistry and western blotting showed that the expressions of Rock - I and MYPT - 1 were significantly up - regulated in the wails of the splenic vein in portal hypertensive patients（P 〈0.01 ）. The two indicators were positively correlated （r = 0.858 ,P 〈0.01 ）. Conclusion The Rock - I expression in the walls of the spleen vein in portal hypertension patients is up - regulated and activated, which suggests that Rho kinase signal transduction may play an important role in the pathological changes in portal hypertensive spleen vein.

关 键 词：Rock—I MYPT-1 门静脉高压症 脾静脉 平滑肌增生 

分 类 号：R657.34[医药卫生—外科学]