MTT法评价猪口蹄疫合成肽疫苗的细胞免疫应答  

Cellullar Immunologic Response of Immunized Swine Following Synthetical Peptide Vaccine by MTT Assay

作　　者：齐鹏 肖进 王楠 巴利民 

机构地区：[1]中牧实业股份有限公司,北京102206

出　　处：《中国兽药杂志》2011年第2期13-15,共3页Chinese Journal of Veterinary Drug

摘　　要：为评价猪口蹄疫合成肽疫苗免疫猪后的细胞免疫应答,用含有E、F两种多肽抗原的猪口蹄疫合成肽疫苗免疫猪,二免后两周采血分离猪外周血淋巴细胞,再用E、F两种合成肽及二者混合物对淋巴细胞刺激培养48 h,用MTT法检测特异性T淋巴细胞增殖反应。结果显示,在抗原E、F混合物浓度为50μg/mL时,抗原对免疫组淋巴结细胞的刺激增殖作用显著高于未免疫对照组(P<0.01)。表明,该猪口蹄疫合成肽疫苗免疫猪能有效引起特异性T淋巴细胞免疫应答。In order to observe the level of cellular immunity,swines were immunized with synthetical peptide vaccine and T lymphocyte proliferate response was investigeted by MTT assay.The synthetical peptide vaccine contained two different peptides E and F.The swine peripheral blood T lymphocyte was collected after the second immunization,and T lymphocyte was cultivated with peptide E、peptide F and their mixture respectively for 48 h.Compared with the control group,the mixtrue with concentration of 50 μg/mL of immunizing group can increase T lymphocyte proliferation and stimulate swine cellular immune response significantly.This record indicated the swine foot and mouth disease synthetical peptide vaccine can stimulate T lymphocyte proliferate response significantly.

关 键 词：MTT法 口蹄疫 合成肽疫苗 T淋巴细胞增殖反应 

分 类 号：S855.3[农业科学—临床兽医学]