雷公藤红素体外抑制人类急性髓系白血病细胞的实验研究  

Inhibitation of proliferation of human acute myelogenous leukemia cells in vitro by celastrol

作　　者：翟亚萍[1] 张茵[1] 马保根[1] 程薇[1] 

机构地区：[1]河南省人民医院血液病研究所,郑州450003

出　　处：《白血病．淋巴瘤》2011年第2期76-78,共3页Journal of Leukemia & Lymphoma

摘　　要：目的研究雷公藤红素体外对人类急性髓系白血病（AML）细胞的体外抑制作用。方法应用四甲基偶氮唑蓝（MTr）法和流式细胞术（FCM）等方法研究雷公藤红素对人类AML细胞的影响。结果MTr实验显示，与空白组比较，培养不同时间后不同浓度组雷公藤红素的细胞增殖抑制率均明显升高，差异有统计学意义（P〈0．01）。FCM检测结果显示，1μmol／L以上浓度雷公藤红素作用不同时间，AML细胞均可出现凋亡现象，凋亡率明显高于不加药物的对照组（P〈0．01）。结论雷公藤红素对AML有明显的抑制作用，其作用可能与其诱导细胞凋亡有关。Objective The current study was designed to investigate the inhibitive effect of eelastrol on the proliferation of acute myelogenous leukemia （AML） cells. Methods The effect of celastrol on AML cells in vitro was examined by MTI＂ assay and flow cytometry （FCM）. Results After the AML primary cells were treated with different concentrations of celastrol, the results were analyzed by MTY. The growth of the cells were significantly inhibited compared with the control group （P 〈0.01）. The results detected by FCM showed that the apoptosis was seen after treated with celastrol with different concentration for different times. The apoptotic rates were significantly higher than that in the control group with statistical significance （P 〈 0.01）. Conclusion Celastrol could inhibit the proliferation of AML cells in vitro, which may be associated with inducing cell apoptosis.

关 键 词：白血病 粒细胞 急性 雷公藤红素 细胞增殖 细胞凋亡 

分 类 号：R285[医药卫生—中药学]