Synthesizing double haploid hexaploid wheat populations based on a spontaneous alloploidization process  

作　　者：Lianquan Zhang Li Zhang Jiangtao Luo Wenjie Chen Ming Hao Baolong Liu Zehong Yan Bo Zhang Huaigang Zhang Youliang Zheng Dengcai Liu Yang Yen 

机构地区：[1]Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China [2]Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China [3]Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA

出　　处：《Journal of Genetics and Genomics》2011年第2期89-94,共6页遗传学报（英文版）

基　　金：funded by the National Basic Research Program(973 Program) of China(No. 2009CB118300);the National Natural Science Foundation of China(No.31071420);Doctoral Fund(No.2.00951E+13);the 100-Talent Program of CAS,the Education Commission and Science and Technology Commission of Sichuan Province and by South Dakota Agricultural Experiment Station

摘　　要：Doubled haploid （DH） populations are useful to scientists and breeders in both crop improvement and basic research. Current methods of producing DHs usually need in vitro culture for extracting haploids and chemical treatment for chromosome doubling. This report describes a simple method for synthesizing DHs （SynDH） especially for allopolyploid species by utilizing meiotic restitution genes. The method involves three steps： hybridization to induce recombination, interspecific hybridization to extract haploids, and spontaneous chromosome doubling by selfing the interspecific Fis. DHs produced in this way contain recombinant chromosomes in the genome（s） of interest in a homogeneous background. No special equipment or treatments are involved in the DH production and it can be easily applied in any breeding and/or genetic program. Triticum turgidum L. and Aegilops tauschii Coss, the two ancestral species of common wheat （Triticurn aestivum L.） and molecular markers were used to demonstrate the SynDH method.Doubled haploid （DH） populations are useful to scientists and breeders in both crop improvement and basic research. Current methods of producing DHs usually need in vitro culture for extracting haploids and chemical treatment for chromosome doubling. This report describes a simple method for synthesizing DHs （SynDH） especially for allopolyploid species by utilizing meiotic restitution genes. The method involves three steps： hybridization to induce recombination, interspecific hybridization to extract haploids, and spontaneous chromosome doubling by selfing the interspecific Fis. DHs produced in this way contain recombinant chromosomes in the genome（s） of interest in a homogeneous background. No special equipment or treatments are involved in the DH production and it can be easily applied in any breeding and/or genetic program. Triticum turgidum L. and Aegilops tauschii Coss, the two ancestral species of common wheat （Triticurn aestivum L.） and molecular markers were used to demonstrate the SynDH method.

关 键 词：ALLOPOLYPLOID Doubled haploid Unreduced gametes 

分 类 号：S512.1[农业科学—作物学] Q348[生物学—遗传学]