Identification of Pns12 as the second silencing suppressor of Rice gall dwarf virus  

作　　者：WU JianGuo WANG ChunZheng DU ZhengGuo CAI LiJun HU MeiQun WU ZuJian LI Yi XIE LianHui 

机构地区：[1]Institute of Plant Virology, Fujian Agriculture and Forestry University, Key Laboratory of Plant Virology of Fujian Province, Fuzhou 350002, China [2]Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China

出　　处：《Science China(Life Sciences)》2011年第3期201-208,共8页中国科学（生命科学英文版）

基　　金：supported by the National Basic Research Program of China(Grant No. 2010CB126203);the National Transgenic Major Program(Grant Nos. 2009ZX08009-044B and 2009ZX08001-018B);the National Natural Science Foundation of China(Grant No. 30970135);the Fujian Province Education Department(Grant No. JB08078);Specialized Research Fund for the Ministry of Agriculture(Grant No. nyhyzx07-051)

摘　　要：RNA silencing is a conserved mechanism found ubiquitously in eukaryotic organisms.It has been used to regulate gene expression and development.In addition,RNA silencing serves as an important mechanism in plants' defense against invasive nucleic acids,such as viruses,transposons,and transgenes.As a counter-defense,most plants,and some animal viruses,encode RNA silencing suppressors to interfere at one or several points of the silencing pathway.In this study,we showed that Pns12 of RGDV (Rice gall dwarf virus) exhibits silencing suppressor activity on the reporter green fluorescent protein in transgenic Nicotiana benthamiana line 16c.Pns12 of RGDV suppressed local silencing induced by sense RNA but had no effect on that induced by dsRNA.Expression of Pns12 also enhanced Potato virus X pathogenicity in N.benthamiana.Collectively,these results suggested that RGDV Pns12 functions as a virus suppressor of RNA silencing,which might target an upstream step of dsRNA formation in the RNA silencing pathway.Furthermore,we showed that Pns12 is localized mainly in the nucleus of N.benthamiana leaf cells.RNA silencing is a conserved mechanism found ubiquitously in eukaryotic organisms.It has been used to regulate gene expression and development.In addition,RNA silencing serves as an important mechanism in plants＇ defense against invasive nucleic acids,such as viruses,transposons,and transgenes.As a counter-defense,most plants,and some animal viruses,encode RNA silencing suppressors to interfere at one or several points of the silencing pathway.In this study,we showed that Pns12 of RGDV （Rice gall dwarf virus） exhibits silencing suppressor activity on the reporter green fluorescent protein in transgenic Nicotiana benthamiana line 16c.Pns12 of RGDV suppressed local silencing induced by sense RNA but had no effect on that induced by dsRNA.Expression of Pns12 also enhanced Potato virus X pathogenicity in N.benthamiana.Collectively,these results suggested that RGDV Pns12 functions as a virus suppressor of RNA silencing,which might target an upstream step of dsRNA formation in the RNA silencing pathway.Furthermore,we showed that Pns12 is localized mainly in the nucleus of N.benthamiana leaf cells.

关 键 词：Rice gall dwarf virus Pns12 silencing suppressor 

分 类 号：Q756[生物学—分子生物学] S435.111.4[农业科学—农业昆虫与害虫防治]