Identification and expression of amphioxus AmphiSmad1/5/8 and AmphiSmad4  

作　　者：YU XueSong LI JianWei LIU Hui LI XiaoDan CHEN ShangWu ZHANG HongWei XU AnLong 

机构地区：[1]State Key Laboratory of Biocontrol, Open Laboratory for Marine Functional Genomics of State High- Tech Development Program, Guangdong Key Laboratory of Pharmaceutical Functional Genes, College of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China [2]Institute of Developmental Biology, College of Life Sciences, Shandong University, Jinan 250100, China

出　　处：《Science China(Life Sciences)》2011年第3期220-226,共7页中国科学（生命科学英文版）

基　　金：supported by the National Basic Research Program of China(Grant No. 2007CB815800);the National High Technology Research and Development Program of China(Grant No. 2006330004104456);the National Natural Science Foundation of China(Grant Nos. 30300264,30270693 and 30570967)

摘　　要：Smad family proteins are identified as intracellular signal mediators of the TGF-β superfamily.In this study,we identified two novel members of the Smad family,termed as AmphiSmad1/5/8 and AmphiSmad4,from Chinese amphioxus.Both AmphiSmad1/5/8 and AmphiSmad4 showed a typical domain structure of Smad proteins consisting of conserved MH1 and MH2 domains.Phylogenetic analysis placed AmphiSmad1/5/8 in the Smad1,5 and 8 subgroup of the R-Smad subfamily,and AmphiSmad4 in the Co-Smad subfamily.The spatial and temporal gene expression patterns of AmphiSmad1/5/8 and AmphiSmad4 showed that they may be involved in the embryonic development of notochord,myotome and alimentary canal,and may help to establish the specification of dorsal-ventral axis of amphioxus.Moreover,AmphiSmad1/5/8 and AmphiSmad4 showed extensive distribution in all adult tissues examined,suggesting that these two genes may play important roles in the morphogenesis of a variety of tissues especially notochord and gonad.Smad family proteins are identified as intracellular signal mediators of the TGF-β superfamily.In this study,we identified two novel members of the Smad family,termed as AmphiSmad1/5/8 and AmphiSmad4,from Chinese amphioxus.Both AmphiSmad1/5/8 and AmphiSmad4 showed a typical domain structure of Smad proteins consisting of conserved MH1 and MH2 domains.Phylogenetic analysis placed AmphiSmad1/5/8 in the Smad1,5 and 8 subgroup of the R-Smad subfamily,and AmphiSmad4 in the Co-Smad subfamily.The spatial and temporal gene expression patterns of AmphiSmad1/5/8 and AmphiSmad4 showed that they may be involved in the embryonic development of notochord,myotome and alimentary canal,and may help to establish the specification of dorsal-ventral axis of amphioxus.Moreover,AmphiSmad1/5/8 and AmphiSmad4 showed extensive distribution in all adult tissues examined,suggesting that these two genes may play important roles in the morphogenesis of a variety of tissues especially notochord and gonad.

关 键 词：AMPHIOXUS SMAD AmphiSmad1/5/8 AmphiSmad4 expression patterns ORGANOGENESIS dorsal-ventral patterning 

分 类 号：Q959.287[生物学—动物学] Q257