Specific cellular immune responses in mice immunized with DNA,adeno-associated virus and adenoviral vaccines of Epstein-Barr virus-LMP2 alone or in combination  

作　　者：WANG Zhan YANG SongMei ZHOU Ling DU HaiJun MO WuNing ZENG Yi 

机构地区：[1]State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China [2]Laboratory Department, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China

出　　处：《Science China(Life Sciences)》2011年第3期263-266,共4页中国科学（生命科学英文版）

基　　金：supported by the National High Technology Research and Development Program of China(Grant No. 2006AA02A229)

摘　　要：Cellular immune responses,particularly those associated with CD3+CD8+ cytotoxic T lymphocytes (CTL),are critical factors in controlling viral infection.Nasopharyngeal carcinoma (NPC) is closely associated with persistent Epstein-Barr virus (EBV) infection.NPC vaccine studies have focused on enhancing specific antiviral CTL responses.In this study,three vaccines capable of expressing the EBV-latent membrane protein 2 (LMP2) (a DNA vector,an adeno-associated virus (AAV) vector,and a replication-defective adenovirus serotype 5 (Ad5) vector) were respectively used to immunize female Balb/c mice (4-6 weeks old) at weeks 0,2 and 4,either alone or in combination.Our results suggest that combined immunization with DNA,AAV,and adenovirus vector vaccines induced specific cellular immunity more effectively than any of these vectors alone or a combination of two of the three,constituting a sound vaccine strategy for the prevention and treatment of NPC.Cellular immune responses,particularly those associated with CD3^＋CD8^＋ cytotoxic T lymphocytes （CTL）,are critical factors in controlling viral infection.Nasopharyngeal carcinoma （NPC） is closely associated with persistent Epstein-Barr virus （EBV） infection.NPC vaccine studies have focused on enhancing specific antiviral CTL responses.In this study,three vaccines capable of expressing the EBV-latent membrane protein 2 （LMP2） （a DNA vector,an adeno-associated virus （AAV） vector,and a replication-defective adenovirus serotype 5 （Ad5） vector） were respectively used to immunize female Balb/c mice （4-6 weeks old） at weeks 0,2 and 4,either alone or in combination.Our results suggest that combined immunization with DNA,AAV,and adenovirus vector vaccines induced specific cellular immunity more effectively than any of these vectors alone or a combination of two of the three,constituting a sound vaccine strategy for the prevention and treatment of NPC.

关 键 词：EBV-LMP2 DNA vaccine recombinant adenovirus vaccine recombinant adeno-associated virus vaccine combinatorial immunization specific cell-mediated immune responses 

分 类 号：Q782[生物学—分子生物学] S852.4[农业科学—基础兽医学]