Phylogenetic relationships within the genus Aspidisca(Protozoa,Ciliophora,Euplotida) revealed by ITS1-5.8S-ITS2 region sequences  

作　　者：黄洁 伊珍珍 苗苗 AL-RASHEID KhaledA.S 宋微波 

机构地区：[1]Laboratory of Protozoology, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China [2]Laboratory of Protozoology, Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, South Chtna Normal University, Guangzhou 510631, China [3]Zoology Department, King Saud University, Riyadh 11451, Saudi Arabia

出　　处：《Chinese Journal of Oceanology and Limnology》2011年第2期277-283,共7页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No. 41006098);a joint grant from the Center of Excellence in Biodiversity,King Saud University;the 111 project (No.B08049)

摘　　要：Abstract The internal transcribed spacer regions （ITS1 and ITS2） and 5.8S rRNA genes were sequenced in six populations of four Aspidisca species, namely A. leptaspis, A. orthopogon, A. rnagna and A. aculeata. Phylogenetic trees were constructed by means of Bayesian inference （BI）, Maximum Parsimony （MP）, Neighbor-Joining （NJ）, and Maximum Likelihood （ML） to assess the inter- and intra-species relationships within the genus Aspidisca. All trees show similar topologies with stable supports and indicate that： （1） four well known groups, i.e., Oligotrichia, Stichotrichia, Choreotrichia and Hypotrichia, are distinctly outlined within the class Spirotrichea, and all are monophyletic other than Hypotrichia; （2） members of Aspidisca can be distinguished well, based on the ITSI-5.SS-ITS2 region sequences, and A. leptaspis and A. magna shared a closer relationship than other species; （3） Aspidisca and Euplotes branch early in the subclass Hypotrichia. To compare the phylogenetic relationships based on different genes, SSU rRNA trees were also constructed with nearly the same species inclusion, which revealed different topologies of inter-species, inter-genera and inter-subclasses.The internal transcribed spacer regions(ITS1 and ITS2) and 5.8S rRNA genes were sequenced in six populations of four Aspidisca species,namely A.leptaspis,A.orthopogon,A.magna and A.aculeata.Phylogenetic trees were constructed by means of Bayesian inference(BI),Maximum Parsimony(MP),Neighbor-Joining(NJ),and Maximum Likelihood(ML) to assess the inter-and intra-species relationships within the genus Aspidisca.All trees show similar topologies with stable supports and indicate that:(1) four well known groups,i.e.,Oligotrichia,Stichotrichia,Choreotrichia and Hypotrichia,are distinctly outlined within the class Spirotrichea,and all are monophyletic other than Hypotrichia;(2) members of Aspidisca can be distinguished well,based on the ITS1-5.8S-ITS2 region sequences,and A.leptaspis and A.magna shared a closer relationship than other species;(3) Aspidisca and Euplotes branch early in the subclass Hypotrichia.To compare the phylogenetic relationships based on different genes,SSU rRNA trees were also constructed with nearly the same species inclusion,which revealed different topologies of inter-species,inter-genera and inter-subclasses.

关 键 词：marine ciliates Aspidisca PHYLOGENY internal transcribed spacer 5.8S rRNA 

分 类 号：Q951.3[生物学—动物学]