缬草提取物对胆汁酸代谢及肝损伤影响的实验研究  

Effects of Veleriana Extracts on Bile Acid Metabolism and Liver Injury

作　　者：徐沙丽[1] 李中明[1] 李哈妮[1] 薛存宽[1] 

机构地区：[1]华中科技大学同济医学院附属梨园医院神经内科,武汉430077

出　　处：《医药导报》2011年第3期298-301,共4页Herald of Medicine

摘　　要：目的探讨缬草提取物对高胆固醇-高脂肪-高糖膳食所致的胆汁-胆汁酸代谢变化及肝损害的影响。方法①大耳白种家兔24只,随机分为3组,每组8只。治疗组和对照组给予高胆固醇-高脂肪-高糖饲料。治疗组每天灌胃缬草提取物30 mg.kg-1,对照组灌胃等容积0.9%氯化钠溶液,正常组自由进食标准颗粒饲料,同时每天灌胃等容积0.9%氯化钠溶液。观察实验兔的血清丙氨酸氨基转移酶(ALT)、血总胆固醇(TC)及总胆汁酸(TBA)含量、胆汁的流量及其TC-磷脂-TBA含量、胆囊结石形成情况、肝组织学检查以及主动脉壁粥样斑块观察。②Wistar大鼠24只,随机分为3组,每组8只。治疗组和对照组给予高胆固醇-高脂肪-高糖饲料,正常组自由进食基础饲料。治疗组每天灌胃缬草提取物50 mg.kg-1,对照组则每天灌胃等容0.9%氯化钠溶液。观察血清TC、TBA及肝组织学检查。结果①家兔治疗组胆总管胆汁流量及固形物含量较对照组显著增加(P<0.01),胆汁中的TBA含量明显增加(P<0.05),血清TBA较对照组明显降低(P<0.05),胆囊内凝结物形成率明显低于对照组(P<0.05)、肝损伤较对照组显著减轻。②大鼠治疗组血清TBA较对照组降低(P<0.05),肝损伤较对照组减轻。结论缬草提取物可以增加胆汁中TBA含量,提高TBA/TC结石形成,降低血清TBA浓度,明显减轻肝损伤。Objective To explore the effects of valerian extracts on bile acid metabolism and liver damage caused by high cholesterol-high sugar-high fat diet. Methods ①24 white rabbits with big ears were randomly divided into 3 groups（n=8 each）.The rabbits were fed with high cholesterol-high fat-high sugar diet except those in the normal group with pellet feed.The treatment group was intragastrically given with valeriana extracts 30 mg·kg-1 daily.The serum pyruvate aminotransferase（ALT）,serum total cholesterol（TC）,total bile acid（TBA） content,bile flow and TC-phospholipids-TBA content,gallstone formation,liver histological test and aortic plaque-like porridge were analyzed later.②24 Wistar rats were randomly divided into 3 groups（n= 8）,which were fed with high cholesterol-high fat-high sugar diet,except for those in the normal group with basal diet.The treatment group were fed with valerianaextracts 50 mg·kg-1 daily,the control group was given with the equal volume of 0.9% sodium chloride solution.The serum TC,TBA and liver histology were observed. Results ①Compared with the control group,common bile duct flow and solids content were increased significantly（P0.01） in the treatment group,TBA concentration in bile was elevated obviously（P0.05）,serum TBA was predominantly lower（P0.05）,gallstone formation was significantly less（P0.05）,liver injury was significantly alleviated.②The serum TBA in rats was dropped（P0.05） in the treatment group compared with that in the control,and the liver injury was attenuated. Conclusion Valeriana extracts could increase the TBA content of bile,improve TBA/TC stone formation,lower the serum TBA concentration and significantly reduce the liver injury.

关 键 词：缬草提取物 胆汁酸代谢 损伤 肝 

分 类 号：R282.71[医药卫生—中药学] R285.5[医药卫生—中医学]