分子印迹在线富集-化学发光法测定葡萄酒和虎杖提取物中的白藜芦醇  

Molecularly imprinted on-line solid-phase extraction combined with chemiluminescence for the determination of resveratrol

作　　者：时作龙 章竹君[2] 唐虹 赵琼 杨曦 陈渝 

机构地区：[1]兵器工业卫生研究所,西安710065 [2]陕西师范大学化学与材料科学院,西安710062

出　　处：《分析试验室》2011年第3期5-8,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(30470886)资助

摘　　要：制备了白藜芦醇的分子印迹聚合物,用聚四氟乙烯管作为微固相萃取柱,连接在流动注射系统的八通阀上,对白藜芦醇进行富集和分离;经甲醇和乙酸混合洗脱液(9∶1,V/V)在线洗脱后与酸性KMnO4发生化学发光反应。测定白藜芦醇的线性范围2.5×10-7～6.1×10-5g/mL,方法的检出限为(3σ)8×10-8g/mL,11次平行测定1.5×10-6g/mL的白藜芦醇溶液的化学发光强度相对标准偏差为2.0%。The molecularly imprinted particles were synthesized using resveratrol as the template.As a solid-phase extraction micro-column,a polytetrafluoroe-thylene tube was connected to the sampling loop of the eight-way injection valve for the preconcentration and extraction of resveratrol.The eluent was used as the carrier to extract the adsorbed resveratrol and then reacted with potassium permanganate in the flow cell to produce CL.The chemiluminescence intensity is correlated linearly with resveratrol concentration in the range of 2.5×10-7～6.1×10-5 g/mL with a detection limit of（3σ） 8×10-6 g/mL.The relative standard deviation is 2.0% for 1.5×10-6 g/mL of resveratrol（n=11）.

关 键 词：分子印迹 化学发光 白藜芦醇 

分 类 号：O657.3[理学—分析化学]