基于聚亚甲基蓝-碳纳米管复合膜固载血红蛋白的H_2O_2生物传感器  

A hydrogen peroxide biosensor based on hemoglobin/Poly (methylene blue)-carbon nanotube modified electrode

作　　者：杨怀成[1,2] 罗胜联[2] 魏万之[2] 

机构地区：[1]桂林师范高等专科学校化学系,桂林541001 [2]湖南大学化学生物传感与计量学国家重点实验室,长沙410082

出　　处：《分析试验室》2011年第3期63-67,共5页Chinese Journal of Analysis Laboratory

基　　金：高等学校博士学科点专项科研基金(20060532006);广西教育厅科研项目(201010LX577);桂林师范高等专科学校科研基金(GLSZ200911)资助

摘　　要：采用吸附和电化学聚合修饰方法,制得了聚亚甲基蓝-碳纳米管聚合膜玻碳电极(PMB-MWNTs/GCE),再将血红蛋白(Hb)固定在PMB-MWNTs/GCE表面,制备了稳定的Hb/PMB-MWNTs/GCE的H2O2生物传感器,并用循环伏安法对修饰电极的生物电催化行为进行了表征。研究结果表明,固定在PMB-MWNTs/GCE电极表面的Hb能进行有效和稳定的直接电子转移反应,对低浓度H2O2具有良好的催化还原响应。在优化的实验条件下,该生物传感器对H2O2响应的线性范围为1.0×10-6～2.0×10-4mol/L,检出限为0.4×10-6mol/L。修饰的生物传感器可应用于样品中H2O2的直接测定。Hemoglobin（Hb） immobilized on the surface of the poly（methelene blue）-multi-walled carbon nanotube modified glassy carbon electrode（Hb/PMB-MWNTs/GCE） can undergo effective and stable direct electron transfer reaction.A H2O2 biosensor based on hemoglobin/poly（methelene blue）-multi-walled carbon nanotubes polymer film modified electrode was prepared.The electrochemical behavior of the modified electrode was investigated.Hb on the H2O2 biosensor can carry out persistent and direct electron transfer reaction,and preserve electrocatalytic activity to reduce H2O2.The linearity range of the biosensor for H2O2 was 1.0×10-6 ～ 2.0×10-4 mol/L,and the detection limit was 0.4×10-6 mol/L.The biosensor was applied to direct determination of H2O2,and the result was credible and satisfactory.

关 键 词：血红蛋白 亚甲基蓝 碳纳米管 H2O2 生物传感器 

分 类 号：O657.1[理学—分析化学]