1,2-萘醌-4-磺酸钠体系分光光度法测定头孢他啶  

Spectrophotometric determination of ceftazidime by using sodium 1,2-naphthoquinone-4-sulfonate

作　　者：范秉琳[1,2] 张西军[1] 张振轩[1] 李全民[1] 

机构地区：[1]河南师范大学化学与环境科学学院,河南省环境污染控制重点实验室,新乡453007 [2]河南新乡医学院基础医学院,新乡453003

出　　处：《分析试验室》2011年第3期104-106,共3页Chinese Journal of Analysis Laboratory

摘　　要：建立了用1,2-萘醌-4-磺酸钠(NQS)作为显色剂分光光度法测定头孢他啶(ceftazidime)的新方法。在pH13.0缓冲溶液中,NQS能直接与头孢他啶发生亲核取代反应生成红褐色化合物,其最大吸收波长为484 nm。头孢他啶在2.98～127.3 mg/L浓度范围内与吸光度呈良好线性关系,线性回归方程A=0.01765+0.00619ρ(mg/L),相关系数r=0.9987,检出限和RSD分别为0.87 mg/L,1.9%。本法能直接用于注射剂中头孢他啶含量的测定,回收率在98.4%～101.8%。A new method is established to determine ceftazidime by using sodium 1,2-naphthoquinone-4-sulfonate（NQS） as a chromogenic reagent.A russety product can be formed owing to the nucleophilic substitution reaction between ceftazidime and NQS in pH=13.00 buffer solution.The maximal absorption wavelength of the product is 484 nm.Absorbency is linear with the concentration of ceftazidime in the range of 2.98～127.3 mg/L,and the regression equation is A=0.01765＋0.00619c（mg/L）,with a correlation coefficient of 0.9987.The detection limit and the RSD are 0.87 mg/L and 1.9%,respectively.The average recoveries are in the range of 98.4%～101.8%.The study indicated that the method could be applied to the determination of ceftazidime in injections.

关 键 词：1 2-萘醌-4-磺酸钠 头孢他啶 分光光度法 

分 类 号：O657.3[理学—分析化学]