高效液相色谱法测定金莲清热泡腾片中靛玉红的含量  

Determination of indirubin in Jinlianqingre effervescent tablets by HPLC

作　　者：许秀娟 谢秉湘 李志梅 

机构地区：[1]上海市浦东新区周浦医院,上海201318 [2]浙江温州市药品检验所,温州325028

出　　处：《海峡药学》2011年第2期39-40,共2页Strait Pharmaceutical Journal

摘　　要：目的建立金莲清热泡腾片中靛玉红的HPLC含量测定方法。方法色谱柱为安捷伦Eclipse-XDB-C18柱(250mm×4.6mm,5μm),甲醇-水(75∶25)为流动相,流速:1.0mL.min-1,检测波长为289nm,柱温:35℃。结果靛玉红在2.07～41.44ng范围内线性关系良好(r=1)。平均回收率为98.3%,RSD0.8%(n=6)。结论方法准确可靠,可作为金莲花清热泡腾片的质量控制方法。OBJECTIVE To establish a HPLC method for the determination of indirubin in Jinlianqingre effervescent tablets.METHODS The Eclipse-XDB-C18column（250 mm×4.6 mm,5μm）was used,the mobile phase was methanol-water（75∶25）;the detection wavelength was 289 nm,the column temperature was 35 ℃,the flow rate was 1.0mL·min-1.RESULTS Thelinear range of indirubin was 2.07～41.44ng（r=1）,the average recovery was 98.3%,RSD 0.8%（n=6）.CONCLUSION The method is accurate and reliable,it can be used for the quality control method of Jinlianqingre effervescent tablets.

关 键 词：金莲清热泡腾片 高效液相色谱法 靛玉红 

分 类 号：R927.2[医药卫生—药学]