基于线粒体DNA控制区(mtDNA D-loop)序列分析瓢鸡的遗传多样性  

The Investigation of Genetic Diversity of Piao Chicken Based on Mitochondrial DNA D-loop Region Sequence

作　　者：贡潘偏抽[1] 刘丽仙[2] 李大林 袁跃云 孙利民 杨润 刘文瑜 刘伟[1] 苗永旺[1] 

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]云南农业职业技术学院畜牧兽医系,云南昆明650212 [3]云南省家畜改良工作站,云南昆明650021 [4]云南省普洱市畜牧工作站,云南普洱665000 [5]云南省普洱市镇沅县畜牧工作站,云南镇沅666500

出　　处：《云南农业大学学报（自然科学版）》2011年第2期211-214,223,共5页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省应用基础研究重点项目(2007C0003Z);云南省应用基础研究计划面上项目(2006C0034M);国家自然科学基金项目(30660024);国家高技术研究发展计划("863"计划)项目(2008AA101001);云南省教育厅科学研究基金项目(09C0197)

摘　　要：采用PCR产物直接测序技术对云南省镇沅县50只瓢鸡样品的线粒体DNA控制区(mtDNA D-loop)第Ⅰ高变区序列进行了分析。结果表明:在所分析的D-loop区序列中(520 bp),T,C,A,G平均含量分别为30.4%,29.6%,27.4%,12.6%;共检测到27个核苷酸多态位点,其中24个为转换,3个为颠换,序列突变率为5.19%;序列存在13种单倍型,单倍型多样度(H)为0.883±0.028,核苷酸多样度(π值)为0.015 03±0.000 99,表明瓢鸡群体内遗传变异较丰富。瓢鸡样品可划分为A,B,E,F,G等5个世系,在NJ无根系统树上聚为5大枝,揭示瓢鸡在遗传组成上具有5个母系血统来源。In the present paper,the PCR products of the mitochondrial D-loop sequences（520 bp in length） of 50 individuals from Piao chicken（which have no pygostyle） of Zhenyuan County,Yunnan province,China,were directly sequenced.The results revealed that the average percentage values of base composition of T,C,A and G in the sequences were 30.4%,29.6%,27.4% and 12.6%,respectively.In the samples assayed,27 mutation sites included 24 transitions and 3 transversions viz.5.19% mutation rate and 13 haplotypes were found,haplotype diversity（H） was 0.883±0.028 and nucleotide diversity（π value） was 0.015 03±0.000 99.Phylogenetic analysis by using the former study and the unrooted NJ method showed that all the sequences were clustered in 5 major clades.The results in this study indicated that the genetic variability in Piao chicken population was high and there were 5 maternal origins to this chicken.

关 键 词：瓢鸡 线粒体DNA D—loop 单倍型 遗传变异 核苷酸多样度 

分 类 号：S831.2[农业科学—畜牧学]