灯盏花素注射液的生物活性测定方法的建立  

Bioactivity assay of breviscapine injection

作　　者：欧阳杰湖[1] 潘善庆[1] 陈子渊[1] 

机构地区：[1]湖南省药品检验所,长沙410001

出　　处：《中南药学》2011年第2期103-107,共5页Central South Pharmacy

基　　金：湖南省科技计划一般项目(湘财教指[2009]42号)

摘　　要：目的采用生物活性测定法加强控制灯盏花素注射液的质量。方法取清洁级大鼠,体重180200 g,雄性,用YLS-14B血栓生成仪,将刺激电流控制在1 mA,电刺激时间定为3 min,建立大鼠单侧颈总动脉血栓形成实验动物模型来评价灯盏花素注射液活血化瘀的功能。结果比较大鼠颈总动脉单侧血栓全阻塞时间,灯盏花素注射液给药组与模型对照组有显著差异（P〈0.05）,同时延长率〉60%,灯盏花素注射液的生物活性符合规定。结论灯盏花素注射液的生物活性动物模型建立成功,可用于测定灯盏花素注射液的生物活性。Objective To establish a bioactivity assay to enhance the quality control of breviscapine injection.Methods The determination was carried out with clean male rats（body weight,180-200 g）,using YLS-14B thrombus form meter（current flow controlled at 1 mA,stimulus duration 3 min）.Results The time of half arteria carotis communis thrombus hol-blockage was compared.The bioactivity assay of breviscapine injection met the requirements.Significant difference was found between the medication administration team and the control group（P〈0.05）,with lasting rate〉60%.Conclusion This method can be used to control the bioactivity of breviscapine injection.

关 键 词：生物活性测定 活血化瘀 血栓全阻塞时间 灯盏花素注射液 

分 类 号：R965[医药卫生—药理学]