萝卜蚜新蚜虫疠霉的分离鉴定及其致病性测定  

Isolation and Identification of Pandora neoaphidis and Its Pathogenicity Against Lipaphis erysimi

作　　者：贾春生[1] 

机构地区：[1]韶关学院英东生物工程学院,广东韶关512005

出　　处：《安徽农业科学》2011年第3期1385-1386,1392,共3页Journal of Anhui Agricultural Sciences

基　　金：韶关学院重点项目(200402)

摘　　要：[目的]分离鉴定萝卜蚜的病原真菌,并测定其对萝卜蚜的致病性。[方法]采用形态学方法进行病原真菌种类鉴定,利用孢子浴方法对萝卜蚜进行致病性测定。[结果]初生分生孢子,卵形,双囊壁,单核,(24.7±1.4)μm×(10.7±0.9)μm,L/D=2.3±0.2。次生分生孢子形似初生分生孢子,大小为:(18.6±2.1)μm×(13.3±1.3)μm,L/D=1.4±0.2。菌丝段菌丝状,直径为(10.6±0.8)μm。分生孢子梗掌状分枝,直径(10.0±0.9)μm。假囊状体不分枝,基部粗大,直径(19.2±1.7)μm,向端部渐尖,直径(8.0±0.9)μm。假根单菌丝状,基部直径(21.0±3.0)μm,底端具有规则的盘状固着器。休眠孢子未见。该菌对萝卜蚜的致死中量为18.21 cfu/mm2。[结论]感染萝卜蚜的昆虫病原真菌被鉴定为新蚜虫疠霉,该菌对萝卜蚜表现出较强的致病性。[Objective]The study was to isolate and identify the pathogen of Lipaphis erysimi and to determine its pathogenicity against L.erysimi.[Method]Based on the morphological characteristics,the species of pathogenic fungus was identified.Spore shower method was used to deter mine the pathogeni-city against L.erysimi.[Result]Primary conidia,ovoid,bitunicate and uninucleate,（24.7±1.4） μm×（10.7±0.9） μm,L/D=2.3±0.2.Sencondary conidia had the similar shape with the primary ones,（18.6±2.1） μm×（13.3±1.3） μm,L/D =1.4±0.2.Hyphal fragment was like mycelium with the diameter of（10.6±0.8） μm.Conidiophores had palmate branch with the diameter of（10.0±0.9） μm.False cystidia was not branched,which had rough base with the diameter of（19.2±1.7） μm,and gradually became more angular towards the apex with the diameter of（8.0±0.9） μm diameter at tips.Rhizoid was like monohyphal shape with the diameter of（21.0±3.0） μm at base,the terminal apex had regular discoid holdfast.No dormancy spores was observed.The lethal dose of the pathogen against L.erysimi was 18.21 cfu/mm2.[Conclusion]The entomopathogenic fungus against L.erysimi was identified to be Pandora neoaphidis,and the pathogen was confirmed to have strong pathogenicity against L.erysimi.

关 键 词：新蚜虫疠霉 生物测定 萝卜蚜 昆虫病原真菌 虫霉目 

分 类 号：S855.3[农业科学—临床兽医学]