热灭活处理对HIV抗体检测影响的实验研究  

Study on die Effect of HIV Antibody Detection after Heat Inactivation of Samples

作　　者：白立石[1] 佟永力 吴玉华[1] 

机构地区：[1]黑龙江省卫生防疫站,哈尔滨市150036

出　　处：《中国性病艾滋病防治》1999年第4期170-171,共2页Chinese Journal of Std & Aids Prevention and Control

摘　　要：用56℃30min和65℃lh热灭活HIV方法处理标本(包括HIV抗体阳性的和正常的血清、血浆球蛋白的样品),然后用ELISA PA和WB检测HIV抗体.其实验结果与另一组未经处理的同份标本检测结果相同.说明标本热灭活处理后检测HIV抗体不影响实验结果.因此,HTV抗体检测实验室对检样预先热灭活HIV的处理方法是一种简便有效的实验室安全防范措施,可以避免实验室HIV感染的发生.Samples including HIV positive, HIV negative sera and r -globulin were treated at 56C for 30min and at 65C for 1 hour, respectively; then ELISA, PA and WB were performed to detect HIV antibody. A control group that the samples were not heat -treated was set up. The results indicated that heat inactivation did not affect the final outcome for both HIV positive and negative samples. Serum sample had a better result after heat inactivation than globulin. Heat inactivation, therefore, was a good measure to protect laboratory professionals from being infected with HIV in their test work.

关 键 词：热灭活 HIV 检测 

分 类 号：R187.5[医药卫生—流行病学] R373.9[医药卫生—公共卫生与预防医学]