苦参碱对小菜蛾幼虫γ-氨基丁酸受体基因表达的影响  

Effect of matrine on γ-aminobutyric acid receptor gene expression of diamondback moth larvae

作　　者：丁宝力[1] 孟琳琳[1] 王进忠[1] 张志勇[1] 张民照[1] 张鹏飞[1] 丁佳[1] 蒲媛媛[1] 

机构地区：[1]北京农学院植物科学技术学院,北京102206

出　　处：《北京农学院学报》2011年第1期16-19,共4页Journal of Beijing University of Agriculture

基　　金："大学生科学研究与创业行动计划"项目;北京市教育委员会项目(KM200610020007;PXM2008_014207_055164);北京市自然基金资助项目(5043026);北京市科委资助项目(Z080005032508017);国家自然科学基金资助(30971938)

摘　　要：采用实时荧光定量RT-PCR法,分别于4、8、12、16、20和24 h时,检测了不同浓度苦参碱对小菜蛾幼虫体内γ-GABA受体基因的相对表达量。结果表明,在处理4 h时,以为15.625×10-3mg/mL的苦参碱处理小菜蛾,其γ-GABA受体基因的表达量达到最大值,为23.62,在20 h时,γ-GABA受体基因的表达量达到最小值,为11.456。在4、8、12、16和20 h时,125×10-3mg/mL的苦参碱处理小菜蛾幼虫的γ-GABA受体基因的表达量均低于其他的处理,在24 h时,同样浓度处理小菜蛾,其γ-GABA受体基因的表达量高于其他处理,差异显著,在16 h时,同样的浓度处理小菜蛾幼虫γ-GABA受体基因的表达量低于其他处理,达到最小值,为4.345。由此说明苦参碱抑制了小菜蛾体内γ-GABA受体基因的表达。Effect of matrine on γ-aminobutyric acid receptor gene expression of diamondback moth,Plutella xylostella（L.） were investigated.The relative γ-aminobutyric acid receptor gene expression of P.xylostella larvae treated with matrine at 4,8,12,16,20,and 24 h,respectively,was analyzed by real-time quantitative RT-PCR.The results showed that the relative γ-GABA receptor gene expression of P.xylostella larvae treated with 15.625 × 10^-3 mg/mL of matrine reaches its maximum value of 23.620 and a minimum value of 11.456 at 4 h and 20 h,respectively.The relative γ-GABA receptor gene expression of P.xylostella larvae treated with 125×10^-3 mg/mL of matrine was lower than other concentrations at 4,8,12,16 and 20 h,respectively,while the relative γ-GABA receptor gene expression of them at 24 h was higher than other concentrations significantly.The relative γ-GABA receptor gene expression of them at 16 h reached a minimum value of 4.345 which was lower than the other treated concentrations of matrine.It also indicated that the relative γ-GABA receptor gene expression of diamondback moth larvae was inhibited by matrine.

关 键 词：苦参碱 小菜蛾 γ-氨基丁酸受体基因 基因表达 

分 类 号：S482.3[农业科学—农药学]