3种植物DNA提取法中多糖类物质去除效果的研究  

The removal of polysaccharide in three plant DNA extraction methods

作　　者：高洪晓[1] 杨凯[2] 刘建斌[1] 

机构地区：[1]北京农学院园林学院,北京102206 [2]北京农学院农业应用新技术北京市重点实验室,北京102206

出　　处：《北京农学院学报》2011年第1期70-72,共3页Journal of Beijing University of Agriculture

基　　金：北京市教委科技计划面上项目"丁香属植物杂交育种及新品种选育"(KM200510020003)

摘　　要：为探明DNA提取过程中去除多糖的有效方法,以丁香属3种植物为材料,用3种DNA提取方法(普通CTAB法、氯苯法和高盐法)提取丁香属植物基因组DNA。对提取的DNA质量采用琼脂糖凝胶电泳和紫外分光光度计法进行检测,并采用蒽酮硫酸法测定DNA提取过程中多糖含量的变化。结果表明:3种方法的多糖去除率相近且都比较高。高盐法与前两种方法相比对人体和环境危害较小且操作简单,是最适合该属植物的DNA提取方法。该方法采用了专门的去除多糖的步骤,为DNA提取过程中多糖的去除提供了一定的理论依据。To find an efficient method for the removal of polysaccharide in plant DNA extract process,the CTAB,high salt and Chlorobenzene methods were used to extract the genome DNA of three Syringa plants.Agarose gel electrophoresis and ultraviolet spectrophotometer methods were adopted to test the DNA quality,and the concentration changes of polysaccharid in the DNA extraction process were tested with anthraquinone hydrate-sulfuric.The result showed that the removal efficiency of polysaccharide in the three methods were similar.The high salt method was safer and convenienter than the other two methods.The influence of polysaccharide in the high salt methods was removed in a special step.The result provided theoretical basis for the removal of polysaccharide in plant DNA extraction process.

关 键 词：多糖 DNA 高盐法 

分 类 号：S685.26[农业科学—观赏园艺]