拟南芥春化相关基因VRN2的克隆及其植物表达载体的构建  

Cloning Vernalization-Related Gene VRN2 and Construction of its Plant Expression Vector in Arabidopsis thaliana

作　　者：张文惠[1] 洪丽萍[1] 曾碧玉[1] 

机构地区：[1]福建省亚热带植物研究所福建省亚热带植物生理生化重点公共实验室,福建厦门361006

出　　处：《亚热带植物科学》2011年第1期1-4,共4页Subtropical Plant Science

基　　金：福建省自然科学基金项目(2007J0250);福建省省属公益类科研院所基本科研专项(2009R10005-4)

摘　　要：本文利用特异性引物,从拟南芥RNA中提取春化相关基因VRN2 cDNA序列,GenBank登录号AY063047。该基因序列大小为1 354 bp,编码区为1 323 bp,编码氨基酸441个。将克隆片段插入中间载体pBPF?7,经PstI酶切回收带有P35S启动子和nos终止子片段,连接pBI121载体,构建VRN2基因的植物表达载体。In this paper,the vernalization-related Gene VRN2 cDNA from RNA of Arabidopsis thaliana L.,GeneBank accession number AY063047 was successfully cloned.The cDNA sequence of VRN2 was 1 354 bp and its region in encoding was 1323 bp.The protein deduced from VRN2 contained 441 amino acids.The cloned cDNA of VRN2 was introduced into a middle vector pBPF？ 7.The fragment with P35S promoter and nos were digested by PstI,which were connected to pBI121 vector,giving plant espression vector containing VRN2 gene.

关 键 词：春化相关基因 VRN2 春化作用 拟南芥 

分 类 号：Q812[生物学—生物工程]