杜仲多糖抗肝纤维化作用的实验研究  

Protection of Eucommia ulmoides polysaccharide on liver fibrosis

作　　者：周程艳[1] 艾凌艳[1] 王美[1] 王旭[1] 

机构地区：[1]河北联合大学药学院,河北唐山063000

出　　处：《中草药》2011年第2期324-329,共6页Chinese Traditional and Herbal Drugs

基　　金：河北省唐山市科技局科研项目(09130223b)

摘　　要：目的研究杜仲多糖对CCl4致肝纤维化大鼠的保护作用,并探讨其作用机制。方法采用大鼠首次背部sc纯CCl4 5 mL/kg,以后sc 40%CCl4花生油3 mL/kg,共8周,制备大鼠肝纤维化模型。杜仲多糖ig给药8周,测定大鼠肝脏及脾脏指数,检测大鼠血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)的活性及总蛋白(TP)、白蛋白(ALB)水平,计算ALB与球蛋白(GLOB)的比值(A/G);检测血清中透明质酸(HA)、层粘连蛋白(LN)、III型前胶原(PCIII)、IV型胶原(IV-C)水平;检测肝脏组织中超氧化物歧化酶(SOD)的活性、羟脯氨酸(Hyp)、丙二醛(MDA)和谷胱甘肽过氧化酶(GSH-Px)的水平;并检测肝脏中转化生长因子β1(TGF-β1)的表达。结果杜仲多糖能明显对抗CCl4所致的肝纤维化大鼠肝脏、脾脏指数的升高(P<0.01);显著抑制血清中ALT、AST活性的升高(P<0.01),降低血清中HA、LN、PCIII、IV-C、GLOB的量(P<0.01),升高血清中的TP、ALB的量和A/G的值(P<0.01);降低肝组织中MDA的水平和Hyp的量(P<0.01),亦能升高肝组织中SOD的活性和GSH-Px的水平(P<0.01),明显降低肝组织中TGF-β1的表达。杜仲多糖高剂量组抗肝纤维化的效果最好,并呈现明显的剂量依赖性。结论杜仲多糖具有显著的抗肝纤维化作用。Objective To study the protective effect of Eucommia ulmoides polysaccharide（EUP） on liver-fibrosis rats and investigate its mechanism.Methods Models of liver-fibrosis rats induced by carbon tetrachlotide（CCl4） were established by sc injection of pure CCl4（5 mL/kg） and then peanut oil with 40% CCl4（3 mL/kg） to the back of rats for eight weeks.After the models were ig administrated by EUP for eight weeks,the indexes of the liver and spleen in liver-fibrosis rats were calculated;ALT and AST activities and contents of TP and ALB in serum were examined;Meanwhile,the ratio between ALB and GLOB（A/G） was computed;The four levels of HA,LN,PCIII,and IV-C in serum of liver-fibrosis rats were determined.SOD activities and Hyp,MDA,and GSH-Px levels in liver tissue were examined;The expression of transforming growth factor-β1（TGF-β1） in liver was observed.Results EUP could obviously be against the index increasing of the liver and spleen（P0.01） in liver-fibrosis rats induced by CCl4;remarkably inhibit the increasing of ALT and AST activities in serum（P0.01）;decrease the content of HA,LN,PCIII,IV-C,and GLOB in serum（P0.01）;increase the content of TP and ALB,and ratio of A/G in serum（P0.01）;decrease the MDA amd Hyp levels in liver tissue（P0.01）;and improve SOD activities and GSH-Px levels in liver tissue（P0.01）;and decrease the expression of TGF-β1 as well.The best effect of EUP was observed in the high-dose group and inhibition of EUP on fibrosis was in a dose-dependent manner.Conclusion EUP has the remarkable protective effects on liver-fibrosis rats.

关 键 词：杜仲多糖 CCL4 肝纤维化 转化生长因子β1(TGF-β1) 层粘连蛋白(LN) 

分 类 号：R285.5[医药卫生—中药学]