天麻素对终末糖基化产物诱导神经小胶质细胞炎症因子表达的影响  

Effects of gastrodine on expression of inflammatory cytokines in nerve microglia cell induced by advanced glycation end products

作　　者：张媛元 毛瑞阳[2] 杜晓红[2] 刘毅[3] 

机构地区：[1]温州市平阳县第二人民医院肾内科,浙江温州325405 [2]温州医学院第一附属医院干部科,浙江温州325000 [3]温州医学院第一附属医院肾内科,浙江温州325000

出　　处：《中草药》2011年第2期330-334,共5页Chinese Traditional and Herbal Drugs

基　　金：温州市科技局基金资助课题(Y20070010)

摘　　要：目的观察终末糖基化产物(AGEs)对小鼠神经小胶质细胞BV-2炎症因子白细胞介素(IL-1β、IL-6)表达的影响及天麻素的干预效应。方法将体外培养的小鼠小胶质细胞分成对照组、AGEs组、天麻素(12.5、25、50、100 mg/L)组,培养18 h,观察细胞形态变化,酶联免疫吸附法(ELISA)测定培养上清中IL-1βI、l-6水平,逆转录聚合酶链反应(RT-PCR)方法测定IL-1β、IL-6 mRNA表达。结果倒置显微镜下观察,对照组小胶质细胞多为静止期,突起较少,AGEs组多数细胞突起较多,呈阿米巴样,以AGEs质量浓度为300 mg/L时最明显,天麻素干预组细胞突起较AGEs组有所减少,其中以天麻素50 mg/L组最少。与对照组相比,AGEs组细胞培养上清液中IL-1β、IL-6水平,以及细胞内IL-1β、IL-6 mRNA表达明显增加,具有显著差异(P<0.05);与AGEs组相比,天麻素组细胞培养上清液中IL-1β、IL-6水平,以及细胞IL-1β、IL-6 mRNA表达均有所降低,其中天麻素25、50、100 mg/L组差异显著(P<0.05),以天麻素50 mg/L组降低最显著(P<0.01)。结论 AGEs能够诱发神经小胶质细胞产生IL-1β、IL-6,天麻素能部分抑制AGEs对小胶质细胞的诱导作用。Objective To investigate the effects of gastrodine（GAS） on the expression of IL-1β and IL-6 in nerve microglia BV-2 cell of mice induced by different concentrations of advanced glycation end products（AGEs） and GAS intervention.Methods The cultured BV-2 cells of mice were divided into AGEs group,GAS（12.5,25,50,and 100 mg/L） groups,which were cultured for 18 h to observe the changes of cell morphology.The levels of IL-1β and IL-6 in the cell culture supernate were determined by enzyme linked immunosorbent assay（ELISA） and the expressions of IL-1β and mRNA were analysed by reverse transcriptase polymerase chain reaction（RT-PCR）.Results Observed under an inverted microscope,microglia is mostly quiescent and less protruding in control group;Most cell processes were more and amoeba-like in AGEs group,which was most obviously at the concentration of 300 mg/L.Cell processes in GAS groups reduced less than those in AGEs groups,of which the least was in GAS group at concentration of 50 mg/L.Compared with the control group,the levels of IL-1β and IL-6 in supernatants of cell culture in AGEs group and the expressions of IL-1β and IL-6 mRNA increased with significant difference（P0.05）.Compared with AGEs groups,the levels of IL-1β and IL-6 in supernatants of cell culture in AGEs group and the expressions of IL-1β and IL-6 mRNA obviously decreased,there were significant differences in AGE groups at the concentrations of 25,50,and 100 mg/L（P0.05）,among which the decrease was most significant in the AGE group of 50 mg/L（P0.01）.Conclusion AGEs could induce that nerve microglia produce IL-1β and IL-6 and GAS could partly inhibit the induction of AGEs on microglia.

关 键 词：天麻素 终末糖基化产物 神经小胶质细胞 白细胞介素1Β 白细胞介素6 

分 类 号：R285.5[医药卫生—中药学]