分光光度法测定板栗刺壳中的单宁含量  

Quantitative Determination of Tannin in Chestnut Shell Thorns by Spectrophotometry

作　　者：高龙[1] 王全杰[1,2] 许长通[1] 张姝[1] 范怀林[1] 

机构地区：[1]烟台大学化学生物理工学院,山东烟台264005 [2]国家制革技术研究推广中心,山东烟台264005

出　　处：《西部皮革》2011年第6期16-19,共4页West Leather

基　　金：烟台大学大学生科技创新基金资助项目

摘　　要：采用显色分光光度法测定板栗刺壳中单宁的含量的方法。在弱碱性条件下,板栗刺壳中单宁与钨酸钠-磷钼酸形成稳定的蓝色配合物,在766nm处测定显色液的吸光度确定单宁的含量。以单宁酸为标样,单宁量在0.0004～0.002mg/mL范围内服从比耳定律,回归曲线方程Y=47.913X-0.0503,相关系数R2=0.997。Spectrophotometry determination of tannin content in chestnut shell thorns was established with sodium tungstate-phosphomolybdate acid. Under weak alkaline condition, tannin and sodium tungstate- phosphomolybdate acid can form a stable blue complex, whose absorbanee can be measured at 766 nm to determine the tannin concentration. With tannic acid as the standard sample, Beer＇s Law is obeyed in the concentration range of 0.0004-0.002 mg/mL of tannin, the regression equation was Y=47.913X-0.0503 and the correlation coefficient was 0.997.

关 键 词：板栗刺壳 单宁 钨酸钠-磷钼酸 分光光度法 

分 类 号：TQ94[化学工程] TS57[轻工技术与工程—皮革化学与工程]