强致病性沙门菌快速分子诊断技术的建立  

Rapid test of Salmonella strain with high pathogenicity

作　　者：刘华伟[1] 张宏[1] 王庆贺[1] 刘晓叶[1] 王蕊[1] 邱立[1] 

机构地区：[1]西北农林科技大学,陕西杨凌712100

出　　处：《中国兽医学报》2011年第3期378-383,共6页Chinese Journal of Veterinary Science

基　　金：陕西省科学技术研究发展计划项目(2010K01-28);国家级动物科学实验教学示范中心建设资助项目

摘　　要：在已有工作的基础上,利用多重PCR技术进行沙门菌组氨酸转运操纵子hisJ基因、毒力质粒spvR基因和H1抗原fliC基因的特异性扩增,结果发现,5株(鼠伤寒、猪霍乱、都柏林、肠炎、雏沙门菌)常见强致病性沙门菌标准菌株均可检测出hisJ基因(359 bp)和spvR基因(789 bp),并可检测出fliC-c基因(623 bp)或fliC-i基因(537bp);伤寒沙门菌和亚利桑那沙门菌标准菌株检测出spvR基因,验证了伤寒沙门菌、亚利桑那沙门菌已具毒力质粒的报道,揭示它们对人和动物具强致病性。该技术有助于快速诊断强致病性沙门菌,有助于发现新的强致病性沙门菌,并为亚利桑那沙门菌以及常见具H1-c、H1-i抗原沙门菌的血清型鉴定提供辅助依据,可应用于公共卫生、食品卫生和口岸检疫等领域。A multiplex PCR assay was developed and used for detecting hisJ genes,spvR gene and fliC gene in H1of Salmonella,including.S.typhimurium,S.choleraesuis,S.dublin,S.enteritidis S.pullorum standard strains and control bacteria standard strains.The results showed that 5 Salmonella standard strains with high pathogenicity were specifically amplified hisJ（359 bp）and spvR（789 bp）,fliC-c（623 bp） and fliC-i（537 bp） of H1 antigen.Of Salmonella strain with high pathogenicity,S.typhi and S.arizona standard strains also were specifically amplified spvR,which confirmed that S.typhi and S.arizona carry virulence plasmid,and are strong pathogenic strains.The method is useful for detection of new Salmonella strains with high pathogenicity,and for serum diagnosis of S.arizona, S.typhimurium,S.aberdeen and S.choleraesuis from genus level.

关 键 词：强致病性沙门菌 H1抗原 毒力质粒 多重PCR 快速诊断 

分 类 号：S852.61[农业科学—基础兽医学]