鸡组织中磺胺氯吡嗪和磺胺氯哒嗪残留的HPLC检测方法  

Determination of sulfachlor ryraizine and sulfachlorpyridiazine residues in chicken tissue by high performance liquid chromatcgraphy

作　　者：卜仕金[1] 刘兆文[1] 王柄璋[1] 孙雨[1] 汤仁想[1] 蔡健康[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009

出　　处：《中国兽医学报》2011年第3期407-410,415,共5页Chinese Journal of Veterinary Science

基　　金：农业部2009年行业标准制定项目(农财发[2009]105号)

摘　　要：建立了一种鸡组织样品中磺胺氯吡嗪和磺胺氯哒嗪残留量的HPLC测定方法。鸡组织样品中的药物残留经乙腈和丙酮提取,正己烷去脂肪和MCX固相萃取小柱净化。洗脱液经浓缩后,其样品中的药物残留采用HPLC紫外检测器检测,流动相为乙腈/0.017 mol/L磷酸溶液(32/68),紫外检测波长为270 nm。2种磺胺药对照品溶液在0.02～1 mg/L范围内呈良好的线性相关(r>0.999 7)。在50、100、200μg/kg的添加水平上,磺胺氯吡嗪和磺胺氯哒嗪残在鸡组织(肌肉、肝脏和肾脏)中的回收率在70%%～85%之间,日内和日渐变异系数分别小于8%和10%。在该检测条件下,上述两种磺胺药物在鸡组织中的检出限为20μg/kg,定量限为50μg/kg。该检测能够满足鸡组织中磺胺氯吡嗪和磺胺氯哒嗪残留的检测要求。A method was developed for the determination of sulfachlorpyrazine and sulfachlorpyridiazine in chicken tissues by high-performance liquid chromatography.The two sulfonamides residues in chicken tissues were extracted with acetonitrile and acetone.The extracts were defatted with hexane and purified by a positive ion exchanging MCX cartridge.sulfachlorpyrazine and sulfachlorpyridiazine were determined by HPLC on C18 column with ultraviolet detection at 270 nm,and mobile phase was acetonitrile-0.017 mol/L phosphoric acid（32/68）.Under above chromatography conditions,the response for sulfachlorpyrazine and sulfachlorpyridiazine was linear in the range of 0.02-1.0 fig/L（r0.999 7）.Recoveries at fortified level of 50,100 and 200μg/kg in chicken tissues ranged from 70%to 85%,the within-day coefficient variation（C.V） and between-day C.V were less than 8%and 10%respectively.The detection limits were 0.02 mg/kg,the detection quantity were 0.05 mg/kg.This method could meet the demand of residue determination of sulfachlorpyrazine and sulfachlorpyridiazine in chicken tissues.

关 键 词：磺胺氯吡嗪 磺胺氯哒嗪 鸡组织 高效液相色谱 残留检测 

分 类 号：S859.84[农业科学—临床兽医学]