产后子宫内膜炎患牛子宫内膜肥大细胞的研究  

The study on endometrial mast cell in endometritis cow

作　　者：王国卿[1] 王洪海[2] 张乃生[3] 郭梦尧[3] 

机构地区：[1]东北大学生物技术研究所,辽宁沈阳110004 [2]东北农业大学动物医学学院,黑龙江哈尔滨150030 [3]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2011年第3期416-419,共4页Chinese Journal of Veterinary Science

基　　金：中国博士后科学基金资助项目(20080440146;200902492);国家十一五科技支撑计划资助项目(3A4090016201)

摘　　要：本研究旨在探讨子宫内膜肥大细胞在牛子宫内膜炎中的作用。选用产后6～10 d健康及患急性化脓性子宫内膜炎的西门塔尔牛各10头,分别为对照组和试验组,通过ELISA法检测子宫内膜中SP、VIP、组胺浓度,透射电镜观察子宫内膜MC颗粒状态及荧光定量PCR法检测组胺受体H1和H2 mRNA表达情况。结果表明,试验组子宫内膜组织中组胺、SP、VIP含量分别为(80.305±4.002)μg/L、(1 258.06±128.88)ng/L、(615.73±70.50)ng/L,而对照组分别为(39.204±4.278)μg/L、(308.12±9.72)ng/L,(1 667.34±153.4)ng/L,试验组与对照组相比差异均极显著(P<0.01);试验组子宫内膜固有层MC颗粒数量减少、分布和浓度不均、有空泡形成,对照组子宫内膜固有层MC颗粒较多、分布均匀;试验组中组胺受体H1 mRNA水平显著低于正常组(P<0.05),组胺受体H2mRNA水平高于正常组,且差异极显著(P<0.01)。结果显示子宫内膜炎时,在神经肽和炎症刺激下使子宫内膜固有层MC脱颗粒大量释放组胺,同时子宫内膜组胺受体H1和H2 mRNA表达失衡,使子宫局部淤血和迟缓,导致子宫免疫力下降,利于病原微生物的繁殖,促进子宫内膜炎的发生发展。Our study focus on investigating the role of endometrial mast cells（MC） in cow endometritis.We selected 6-10 days post-natal Simmental catties,which were health and suffered from acute purulent endometritis as control group and experimental group of 10 each,respectively.The endometrial SP,VIP,histamine concentration were detected by ELISA,the endometrial MC particle state was checked by transmission electron microscopy,and histamine receptor H1 and H2 expression was examined using real-time PCR in the bovine endometrium.The concentration of endometrial SP,VIP,histamine concentration was（80.305±4.002）μg/L,（1 258.06±128.88）μg/L,（615.73±70.50） ng/L in experimental group,and（39.204±4.278）μg/L,（308.12±9.72）μg/L,（1 667.34±153.4） ng/L in control group,respectively,with the significant difference（P0.01）.In control group,inequality of size,homogeneous distribution,round/oval granule were discovered in MC cytoplasm of endometrium lamina propria;in experimental group,MC was degranulated,granule decreased,unevenness the distribution,cavity increment and vacuolization. The expression level of histamine receptor H1 mRNA was lower notably in experimental group than that of control group（P0.05）,but histamine receptor H2 mRNA was higher in experiment group than that of contral group,the significant difference（P0.01） indicating that.Neuropeptide and inflammation may result in MC degran-ulationtc, releasd histamine,and the expressed imbalance of histamine receptor H1 and H2 mRNA in postpartum endometritis, lead to the part of uterus haemostasis and dilatoriness dued to the immune suppression of uterus,pathogenic microorganism multiplication.and lead to the development of endometritis.

关 键 词：牛 子宫内膜炎 肥大细胞 组胺 

分 类 号：S858.23[农业科学—临床兽医学]