胎牛血清对猪孤雌胚早期体外发育的影响  

Effect of supplementation of FCS on the development of porcine parthenogenetic embryo in vitro

作　　者：宋雪梅[1,2] 王猛[1,2] 邓星[1,2] 卢晟盛[1,2] 卢克焕[1,2] 

机构地区：[1]广西大学广西亚热带生物资源保护利用重点实验室,广西南宁530004 [2]广西大学动物科技学院,广西南宁530004

出　　处：《中国兽医学报》2011年第3期425-427,432,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30860039);广西科学研究与技术开发计划资助项目(桂科能0815011-6-3)

摘　　要：探讨了在猪孤雌胚体外发育的第3、4、5、6天分别添加胎牛血清(FCS)对孤雌胚发育能力的影响。结果,在体外培养的第5、6天添加10%FCS组得到的囊胚率和囊胚孵化率最高,第5天添加FCS组的囊胚细胞数最多。结果表明,FCS可以促进猪孤雌胚胎的早期体外发育。The effect of supplementation of 10%fetal calf serum（FCS） on the development of pig parthenogenetic embryos in vitro were investigated.FCS was added into NCSU-23 on days 3,4,5 and 6 of in vitro culture of porcine parthenogenetic embryos.The result showed that the groups of supplementation of FCS on days 5 and 6 obtained the highest blastocyst rate and the highest hatching blastocyst rate,and the group of supplementation of FCS on day 5 gained the highest cell numbers of blastocysts.In conclusion,FCS can enhance the in vitro development of porcine parthenogenetic embryos.

关 键 词：猪 卵母细胞 孤雌激活 胚胎培养 胎牛血清 

分 类 号：S828[农业科学—畜牧学]