Bad基因在黄体期绵羊生殖器官中表达的比较研究  

The on comparison of expression of Bad gene in generative organ of sheep at luteal phase

作　　者：葛晨霞[1] 王龙涛[1] 李向军[1] 贾博寅[2] 马磊[1] 姜怀志[1] 

机构地区：[1]吉林农业大学动物科技学院,吉林长春130118 [2]吉林大学人兽共患病研究所,吉林长春130062

出　　处：《中国兽医学报》2011年第3期428-432,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30871792)

摘　　要：以黄体期小尾寒羊和萨福克羊为研究对象,采用免疫组织化学技术,针对Bad基因在母羊生殖器官卵巢,输卵管和子宫中的表达与定位进行初步研究,在卵巢中Bad基因均在多数黄体细胞中表达;在输卵管宫管结合部、峡部和壶腹部的分泌细胞Bad基因均有不同程度的表达,未发现纤毛细胞Bad阳性细胞;子宫内膜腺体小泡表达Bad阳性,子宫内膜子叶、基质的阳性细胞数极少,阳性细胞呈轻度着色,显弱阳性。利用计算机图像分析技术测量小尾寒羊和萨福克羊Bad基因表达的阳性细胞率和平均光密度,Bad基因在这两种绵羊组织中的表达规律大体相似,也存在一些明显的差异:萨福克羊的黄体细胞较小尾寒羊中的细胞凋亡更明显,萨福克羊输卵管伞部和壶腹部上皮Bad阳性细胞率均高于小尾寒羊;小尾寒羊子宫内膜子叶和基质Bad阳性细胞率和平均光密度均显著高于萨福克(P<0.01)。结果表明,小尾寒羊和萨福克羊生殖器官中细胞凋亡的差异与Bad基因表达的差异,可能是造成两者繁殖力高低差异的基础。During luteal phase（LP） immunohistochemistry was used to confirm and locate apoptosis cells and to detect the expression of Bad in major reproductive organs（ovaries,oviduct and uterus） of Small-tails Han Sheep （SHS） and Suffolk Sheep（SS）,which have high and low productivity,respectively.Bad gene was expressed in most of luteal cells.secretory cells and part of ciliated cell in fimbriaend of fallopian tube,ampullae and isthmusthe epithelia at luteal phase of submited positiveness,ciliated cell was stained strongly at luteal phase.The expression inten difference was unsharpness in different position of fimbriae tubae at contemporaneous.Staining was moderate with Bad positive cells in fimbriaend of fallopian tube,ampullae and isthmusthe epithelia.Staining was light with Bad positive cells in endometrial gland and endometrium cotyledon.Average optical density and average positive area were measured by computator image analysis software in different of position between Small-tails Han Sheep（SHS） and Suffolk Sheep（SS） but also had several visible differences in reproductive organs.The ratio of positive cells and AO of endometrium cotyledon stroma of SHS was higher significantly than SS in LP（P0.01）.The result showed that the difference of apoptosis in reproductive organs and expression of Bad between SHS and SS maybe is agroundwork which result in of their differential productivity.

关 键 词：小尾寒羊 萨福克羊 黄体期 Bad基因 免疫组织化学 细胞凋亡 

分 类 号：S826[农业科学—畜牧学]