牛黄体中促黄体素受体剪接异构体的表达研究  

Expression of luteinizing hormone receptor splice variants in bovine corpus luteum

作　　者：熊秋宏[1,2] 邓琼[2] 于汪洋[2] 高妍[2] 马腾壑[2] 姜昊[2] 戴立胜[2] 许艳丽[2] 崔香顺[2] 赵玉民[3] 赵志辉[1] 张嘉保[2] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]吉林大学实验动物中心,吉林长春130062 [3]吉林省农业科学院,吉林长春130033

出　　处：《中国兽医学报》2011年第3期440-443,共4页Chinese Journal of Veterinary Science

基　　金：国家科技支撑计划资助项目(2007BAD55B03);"863"计划资助项目(2008AA101010);国家自然科学基金资助项目(30972100)

摘　　要：利用RT-PCR结合测序技术,对牛黄体中促黄体素受体(Luteinizing hormone receptor,LHR)在转录后通过选择性剪接而形成的剪接异构体进行鉴定,并比较不同剪接异构体编码蛋白质氨基酸序列的差异,利用RT-PCR对黄体中的LHR剪接异构体进行定量分析,为研究LHR剪接异构体在黄体中的功能奠定基础。结果表明,牛黄体中LHR通过选择性剪接产生了包括全长mRNA在内的6种剪接异构体,其中第11外显子5′端的266个碱基的缺失会使mRNA因发生移码突变而使翻译提前终止。实时定量PCR结果显示,发生外显子缺失的mRNA的表达量总量是全长mRNA表达量的1.94倍。In this paper,RT-PCR and sequencing technique was employed to identify the splice variants of Luteinzing hormone receptor（LHR） in bovine corpus luteum.The difference of deduced protein sequence of the variants was compared.And the expression level of these splice variants was quantified by RT-PCR.The results indicated that six isoforms of LHR were identified,and variants lacking the first 266 of exon 11 generated a frame shift,and produced a premature codon,.The result of RT-PCR showed that total expression of B,C,D,E and F was 1.94 fold compared with A.

关 键 词：牛 黄体 LHR 剪接 RT-PCR 

分 类 号：S823[农业科学—畜牧学] Q78[农业科学—畜牧兽医]