高效液相色谱-质谱法分析啶菌噁唑在番茄灰霉病菌中的代谢行为  

Metabolism Dynamics and Metabolite of SYP-Z048 in Botrytis Cinerea by High Performance Liquid Chromatography-Mass Spectrometry

作　　者：刘鹏飞[1] 刘屹湘[1,2] 韩平[3] 李健强[1] 刘君丽[4] 刘西莉[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193 [2]云南农业大学植物保护学院,昆明650201 [3]北京农产品质量检测与农田环境监测技术研究中心,北京100097 [4]沈阳化工研究院农药生物测定中心,沈阳110021

出　　处：《分析化学》2011年第3期317-322,共6页Chinese Journal of Analytical Chemistry

基　　金："十一五"国家科技支撑计划(No.2006BAD08A03);2007年教育部新世纪优秀人才支持计划(No.NCET-07-0813);中国农业大学-南京农业大学青年教师开放科研基金(No.CN2007002)资助

摘　　要：采用高效液相色谱-质谱法(HPLC-MS)分析了杀菌剂啶菌噁唑在番茄灰霉病菌液体摇培体系中的代谢动态。啶菌噁唑液相色谱定量检测法的线性回归方程为y=54.5x+11.2(r=0.9997),在2.07～72.34 mg/L范围内,方法回收率和精密度符合农药痕量分析要求。代谢影响因子研究结果显示,培养24 h孢子初萌发阶段的灰霉病菌对药剂代谢能力显著优于后期菌丝生长阶段。啶菌噁唑代谢量与接菌量呈正相关。代谢动态研究表明,在0.05,0.10和0.21 mg/L添加浓度下,啶菌噁唑与灰霉病菌经过44 h的共培养,分别降解为初始浓度的53.8%,58.2%和68.6%,代谢随药剂浓度降低而增快,而在不含菌的培养基中降解率低于0.6%(0.21 mg/L)。药剂处理灰霉病菌培养液经HPLC分离获得4个疑似代谢产物峰,其中化合物M2为分子量257的含氯代谢产物。啶菌噁唑在灰霉病菌菌体中发生了代谢作用,代谢速度受菌龄、接菌量和药剂浓度的影响。High performance liquid chromatography-mass spectrometry was used to study the metabolism of a new fungicide 5-（4-chloro pheny1）-2,3-dimethyl-3-（pyri-dine-3）-oxazoline（SYP-Z048） in Botrytis cinerea.The fungus was cultivated in liquid culture medium with SYP-Z048 and the concentration was determined by high performance liquid chromatography.The linear equation was y=8466.1x-46.3（r=0.9995） in the concentration range of 0.02-0.31 mg/L.Impact factors of metabolism were studied.The results show that SYP-Z048,at the concentration of 0.05,0.10 and 0.21 mg/L,was reduced to 46.2%,41.8% and 31.4% respectively after cultivated with B.cinerea for 44 h,but the degradation rate was less than 0.6% without fungi at 0.21 mg/L.The results indicated that SYP-Z048 was metabolized accelerated at lower dosage.The metabolic rate that affected by young mycelium after cultivated for 24 h was obviously higher than that of the mature one.There was a positive correlation between the quantity reduced after a definite period and the amount of B.cinerea put in medium.HPLC-MS was adopted to analyze the metabolic products of SYP-Z048.There were four possible metabolites peaks in chromatogram,of which the compound M2 is chlorine with a molecule weight of 257.The study showed that SYP-Z048 metabolized in Botrytis cinerea and the metabolize rate was affected by the age and amount of mycelium.

关 键 词：啶菌噁唑 番茄灰霉 代谢 液相色谱-串联质谱 

分 类 号：S436.412[农业科学—农业昆虫与害虫防治] S481.1[农业科学—植物保护]