双水相浮选过程中青霉素的分离行为  

Separation Behavior of Penicillin in Aqueous Two-Phase Flotation

作　　者：毕鹏禹[1] 常林[2] 董慧茹[1] 

机构地区：[1]北京化工大学理学院,北京100029 [2]中国检验检疫科学研究院,北京100123

出　　处：《分析化学》2011年第3期425-428,共4页Chinese Journal of Analytical Chemistry

基　　金：北京化工大学研究生科研创新基金项目(No.09Si002)资助

摘　　要：基于双水相浮选技术(ATPF)分离富集水相中青霉素的方法,研究了双水相浮选过程中青霉素的分离行为。在常温下,2.5 g/L青霉素水溶液300 mL、初始pH 7、(NH4)2SO4浓度350 g/L、浮选溶剂为50%(w/w)PEG1000水溶液10 mL条件下,分别研究了青霉素在双水相浮选过程中的动力学行为和分离后的赋存状态。3种通气流速条件下的动力学研究结果表明,青霉素分离过程存在两个阶段,分别是沉淀浮选阶段和分子吸附浮选阶段,两个阶段均遵守一级动力学;双水相浮选中有机相的红外差谱表明,青霉素以离子形态直接溶解于聚乙二醇相,这是双水相浮选分离效率明显高于传统萃取技术的根本原因。Based on the application of aqueous two-phase flotaion（ATPF） in the separation and concentration of penicillin from aqueous phase,the separation behavior of penicillin in the ATPF process was investigated.The kinetic behavior and the penicillin status in organic phase were studied under the conditions of room temperature,300 mL penicillin aqueous solution of 2.5 g/L,initial pH 7.0,ammonium sulfate concentration of 350 g/L,10 mL polyethyleneglycol（PEG） aqueous solution（50%,w/w）.Using three flow rates,the kinetic results show that there are two stages in the separation process of ATPF：（1） precipitation flotation and（2） molecular adsorption flotation,and both of them follow the fist order kinetics equation.Moreover,the subtractive spectroscopy of organic phase shows that penicillin ion can directly dissolve into PEG phase.This is the reason that the separation efficiency of ATPF is significantly higher than that of the traditional extraction techniques.

关 键 词：双水相浮选 溶剂浮选 双水相萃取 青霉素 分离行为 

分 类 号：TQ465.1[化学工程—制药化工]