不同亚型中重度支气管哮喘患儿白细胞介素-5、免疫球蛋白E水平变化及意义  

Changes of Interleukin-5 and Immunoglobulin E Levels in Moderate to Severe Asthmatic Children with Di-fferent Asthma Subtypes and Their Clinical Significance

作　　者：陈环[1] 檀卫平[1] 李静[1] 麦贤弟[1] 蓝丹[1] 

机构地区：[1]中山大学孙逸仙纪念医院儿科,广州510120

出　　处：《实用儿科临床杂志》2011年第4期267-269,共3页Journal of Applied Clinical Pediatrics

基　　金：广东省自然科学基金(06021301);2009年广东省自然科学基金博士启动项目(9451008901003013)

摘　　要：目的研究IL-5、IgE水平在不同亚型中重度患儿哮喘发病中的变化及意义。方法选取2009年9月-2010年3月于本院儿科哮喘专科门诊就诊的中重度哮喘患儿65例(哮喘组),将哮喘组中急性发作期患儿按诱导痰的细胞分类分为嗜酸性粒细胞(EOS)型哮喘(EA)组及非EOS型哮喘(NEA)组。选取20例健康儿童为健康对照组。采用ELISA法检测不同亚型哮喘患儿血清IgE水平。将哮喘组及健康对照组儿童外周血单个核细胞(PBMC)与CD3单克隆抗体及CD28单克隆抗体共培养,ELISA法检测PBMC在抗体刺激下分泌的IL-5水平。并对IL-5表达水平与IgE水平进行相关性分析,了解IL-5、IgE在不同亚型中重度哮喘患儿中的作用。结果 EA组诱导痰EOS百分比明显高于NEA组(P=0.000)。哮喘组患儿PBMC表达的IL-5水平显著高于健康对照组,急性发作组明显高于临床缓解组,急性发作重度持续组显著高于中度持续组,EA组显著高于健康对照组及NEA组。EA组血清IgE水平显著高于NEA组(P=0.010)。哮喘患儿外周血及EOS诱导痰EOS比例与IL-5、IgE水平呈显著正相关(Pa=0.000);哮喘患儿IL-5水平与IgE水平也呈显著正相关(r=0.482,P=0.001)。结论 IL-5、IgE参与哮喘的发病,IL-5、IgE在EA中的作用较NEA更为突出。IL-5水平与哮喘病情分度密切相关。Objective To investigate the changes and significances of interleukin-5（IL-5） and immunoglobulin E（IgE） levels in children with different subtypes asthma,ranging from moderate to severe cases.Methods Sixty-five moderate to severe asthmatic children（asthma group） and 20 healthy children（healthy control group） were selected from Sep.2008 to Mar.2009 in Department of Pediatrics,Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University in the research.The asthmatic children in the acute stage were divided into 2 groups according to the cell type in induced sputum,one group was labeled as eosinophilic（EOS） asthma（EA）group,and the the other non-EOS asthma（NEA） group.The whole levels of serum IgE of asthmatic children with different subtypes asthma were checked with enzyme linked immunosorbent assay（ELISA）.The vitro peripheral blood mononuclear cells（PBMC） in vitro in both asthmatic groups and healthy control group were cultured with CD3 and CD28 monoclonal antibody collectively.The excretive level of IL-5 in PBMC stimulated by monoclonal antibody was detected with ELISA.A correlation analysis on the metrical level of IL-5 and IgE was carried out in order to investigate the mechanism of IL-5 and IgE in different asthma types.Results The percentages of EOS in the induced sputum in EA group were significantly higher than those in NEA group（P=0.000）.The IL-5 level in PBMC in vitro in asthmatic group was significantly higher than that in the healthy control group.The IL-5 level in acute group was significantly higher than that in remission group,and in the acute group,the IL-5 level in severe persistent group was significantly higher than that in moderate persistent group.Furthermore,the IL-5 level in EA group was significantly higher than that in the healthy control group and NEA group.The IgE level in peripheral blood in EA group was significantly hig-her than that in NEA group（P=0.010）.The percentages of EOS in the peripheral blood and the induced sputum had positive correlation

关 键 词：支气管哮喘 白细胞介素-5 免疫球蛋白E 儿童 

分 类 号：R725.6[医药卫生—儿科]