绍兴地区鲍氏不动杆菌β-内酰胺酶基因型特点与多药耐药关系的研究  

β-Lactamase genotype characteristics of acinetobacter baumannii in Shaoxing and its connection with multi-drug resistance

作　　者：钱小毛[1] 金海勇[1] 糜祖煌[2] 

机构地区：[1]绍兴第二医院检验中心,浙江绍兴312000 [2]无锡市克隆遗传技术研究所,江苏无锡214026

出　　处：《中华医院感染学杂志》2011年第5期847-848,共2页Chinese Journal of Nosocomiology

摘　　要：目的了解绍兴地区鲍氏不动杆菌（ABA）临床分离株β-内酰胺酶基因型的特点，并探讨其与多药耐药的关系。方法用K—B法检测ABA对7类抗菌药物的敏感性，用聚合酶链反应（PCR）检测18种β-内酰胺酶编码基因。结果254株ABA中多药耐药株占47．6%；其中85株ABA基因检测结果：ADC阳性73株，占85．9％，OXA-23群阳性50株，占58．8％，TEM阳性23株，占27．1％，其余基因均阴性；阳性株中单一基因阳性占24．7％，同时有2～3种基因阳性占75．3％。结论绍兴地区ABAβ-内酰胺酶基因型其特点是基因的携带率高、基因型相对集中、基因型别涵盖面广，同时携带≥2种基因的现象是主流。多基因携带和对碳青霉烯类耐药是导致多药耐药的主要原因。OBJECTIVE To study the β-Lactamase genotype characteristics of Acinetobacter baumannii （ABA） and discuss its connection with multidrug resistance. METHODS The drug sensitivity of ABA to 7 kinds of antibiotics was tested by K-B method and 18 kinds of β-Lactamase genes were detected by PCR. RESULTS The percentage of multi-drug resistant strains in 254 strains of ABA was 47.6% and among the genes tested for 85 strains of ABA, 73 strains of ABA（85. 9%）were ADC positive, 50 strains（58.8%） were OXA-23 positive, 23 strains（27. 1%） were TEM positive. No other genotype was found. The percentage of single positive genotype in positive strains was 24.7% ,while the percentage of 2 to 3 kinds of positive genotypes was 75.3X. CONCLUSION The following are the β-Lactamase genotype characteristics of ABA in Shaoxing：high carrying rate, the relative concentration and wide coverage of genotype, carrying two or more kinds of genes. Resistance to carbopenems and carrying with multiple genes are the main reasons to lead to the multi-drug resistance.

关 键 词：鲍氏不动杆菌 多药耐药 Β-内酰胺酶 基因型 

分 类 号：R378[医药卫生—病原生物学]