反硝化聚磷菌富集、筛选及其特性  

Study on the enrichment isolation and characteristics of denitrifying phosphorus removal bacteria

作　　者：贾学斌[1,2] 王强[1] 杜丛[1] 孙静文[1] 姜欣欣[1] 王春丽 马放[1] 

机构地区：[1]哈尔滨工业大学城市水资源与水环境国家重点实验室,哈尔滨150090 [2]黑龙江大学建筑工程学院,哈尔滨150080

出　　处：《哈尔滨工业大学学报》2011年第2期35-39,共5页Journal of Harbin Institute of Technology

基　　金：国家自然科学基金资助项目(50778052);城市水资源与水环境国家重点实验室(哈尔滨工业大学)开放研究项目(QA200905);黑龙江省教育厅科学技术研究(面上)项目计划(11551370)

摘　　要：为进一步探讨反硝化除磷机理和提供脱氮除磷功能菌株,对A2SBR快速富集驯化并筛选其中反硝化聚磷菌功能菌.采用控制A2SBR进水及运行方式对反硝化聚磷菌进行快速富集筛选,并将所筛菌株进行复配研究,为构建脱氮除磷菌剂奠定基础.两段进水和提高注水比的运行方式能使反硝化聚磷菌在反应器中迅速成为优势菌.实验分离得到效果较好的反硝化聚磷菌,通过脂肪酸鉴定其到属.所筛菌株复配效果最好的是b204和16,吸磷率达85.78%,脱氮率为75.02%,相对于单菌株均表现出较好的脱氮除磷效果.采用两段进水和高注水比运行方式对快速富集反硝化聚磷菌较为有效,适当复配方案对提高处理效果有一定效果,b204和菌16组合可以作为潜在脱氮除磷菌剂生物强化菌剂.To investigate the mechanism of denitrifying phosphorus removal and provide functional strains for nitrogen and phosphorus removal,denitrifying phosphorus removal bacteria（DPB） were enriched and isolated from A2SBR.This study made use of controlling the inflow water operation mode of A2SBR for DPB enrichment and screening.The isolated DPB were complex formulated for construction of nitrogen and phosphorus removal functional bacteria.Two-stage inflow water operating mode and high inflow water rate enabled DPB to become rapidly dominant in the reactor.Five DPB strains were isolated and identified by the microbial identification system with fatty acids.The DPB strain and phosphorus accumulating organisms or denitrobactcria were complex formulated,the results showed that the best combination was b204 and 16.Its phosphorus removal rate was 85.78% and its denitrification efficiency was 75.02%,better than the single strains removal rate.Experimental results also showed that DPB could become dominant populations quickly with optimum cultivation conditions and appropriate influent.

关 键 词：生物除磷 富集 聚磷菌 反硝化聚磷菌 

分 类 号：TU992[建筑科学—市政工程]