MMP-2、MMP-9在乳腺导管癌中表达及意义  

Expression and significance of MMP-2,MMP-9 in breast carcinoma

作　　者：苏书娟[1] 邢鲁奇[1] 陈登庭[1] 邓淼[1] 

机构地区：[1]河南科技大学第一附属医院病理科,河南洛阳471003

出　　处：《中国公共卫生》2011年第3期268-270,共3页Chinese Journal of Public Health

基　　金：洛阳市应用技术研究与开发基金(0802021A)

摘　　要：目的研究乳腺导管癌中基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)的表达及与IV型胶原(collagen IV)的关系。方法免疫组化双染法检测60例乳腺导管癌中MMP-2、MMP-9和IV型胶原的表达并分析其相关性及与肿瘤大小、淋巴结转移及人类表皮生长因子受体-2(C-erbB-2)的关系。结果 MMP-2、MMP-9在乳腺导管癌中的阳性表达率分别为75%,80%,明显高于乳腺正常组织的10%;MMP-2、MMP-9在靠近基膜或者将突破基膜处的癌细胞中表达明显增强,MMP-2、MMP-9在肿瘤直径>2 cm(83.9%,90.3%)、有淋巴结转移(87.0%,91.3%)及C-erbB-2阳性组(80.9%,85.1%)的表达率明显高于相应对照组(58.6%,65.5%),(64.9%,67.6%),(46.2%,53.8%);IV型胶原的表达与MMP-2、MMP-9的表达均呈负相关。结论在乳腺导管癌的局部侵袭和远处转移过程中,MMP-2、MMP-9对IV型胶原的降解可能起重要作用;联合检测MMP-2、MMP-9和IV型胶原,可能对判断乳腺癌的浸润转移潜能及评估预后有参考价值。Objective To research the expression and relationship of matrix metalloproteinase-2（MMP-2）,metrix metalloproteinase-9（MMP-9） and collagen IV in breast ductal carcinoma.Methods Immunohistochemistry double staining was used to detect the expression of MMP-2,MMP-9 and collagen IV in 60 cases of breast ductal carcinoma and analyze their correlation.The relations between MMP-2,MMP-9 and breast ductal carcinoma tumor size,lymph node metastasis,and C-erbB-2 were analyzed.Results The positive expression rate of MMP-2,MMP-9 in breast ductal carcinoma was 75% and 80%,respectively,which were significantly higher than the rate of 10% in normal breast tissues.MMP-2 and MMP-9 were expressed more strongly in the cancer cells approaching the basement membrane or breaking through the basement membrane.The positive expressions of MMP-2 and MMp-9 in the tumor diameter2cm,with lymph node metastasis,and C-erbB-2 positive group were 83.9% and 90.3%,87.0% and 91.3%,and 80.9% and 85.1%,which were significantly higher than 58.6% and 65.5%,64.9% and 67.6%,and 46.2% and 53.8% of the correponding control groups.Collagen IV expression and the expression of MMP-2,MMP-9 was negatively correlated.Conclusion The degradation of collagen IV related to MMP-2 and MMP-9 may play an important role in local invasive and distant metastasis of breast ductal carcinoma.The expressions of MMP-2,MMP-9 and collagen IV may have reference value in determining the invasive and metastatic potential of breast carcinoma and evaluating prognosis.

关 键 词：基质金属蛋白酶-2 基质金属蛋白酶-9 乳腺癌 IV型胶原 免疫组织化学 

分 类 号：R655.8[医药卫生—外科学]