蛋白质含量镧(Ⅲ)-铬天青S配合物探针法测定  

Spectrophotometric determination of proteins with lanthanum(Ⅲ)-chrome azurol S complex as spectral probe

作　　者：陈韵[1] 石展望[2] 黄晓敏[1] 

机构地区：[1]韶关学院生物科学系,广东韶关512005 [2]广西民族大学化学与生态工程学院

出　　处：《中国公共卫生》2011年第3期307-308,共2页Chinese Journal of Public Health

基　　金：广东省科技计划资助项目(2005B33001004)

摘　　要：目的用镧（Ⅲ）-铬天青S配合物光谱探针测定生物样品中蛋白质含量。方法在pH4～6的clrak-lubs（KH2PO4-NaOH）缓冲介质中,蛋白质与镧（Ⅲ）-铬天青S配合物发生结合反应,引起配合物溶液褪色及吸光度降低,在一定范围内其吸光度降低与蛋白质浓度成正比。结果牛血清蛋白（BSA）含量在0～50 mg/L范围内遵循比耳定律,配合物表观摩尔吸光系数ε478=3.3×10^5L/（mol.cm）,4 h内稳定。各种蛋白质检出限为0.23～1.12 mg/L,蛋白尿和唾液2种样品回收率分别为92%和105%,相对标准偏差为1.8%,生物体内常见物质基本上不干扰测定。结论该法灵敏度高,稳定性好,抗干扰强,可直接应用于人血清样品及其他生物样品中蛋白质总量的测定。Objective To detect protein in biological samples with lanthanum（Ⅲ）-chrome azurol S complex as a spectral probe.Methods In clark-lubs（KH2PO4-NaOH） buffer at pH 4-6,the absorbance at 478nm of lanthanum（Ⅲ）-chrome azurol S complex decreases when adding protein within 30min at room temperature,and the decrease of absorbance is proportional to the concentration of proteins,which can be used to detect protein.Results The calibration curve for bovine serum albumin was linear up to 50 mg/L.The apparent molar absorptivity of complex at 478 nm was 3.3×10^5L/moL·cm and the complex was stable within 4 hours.The detection limits of various proteins were 0.23-1.12 mg/L.The detection recoveries of albuminuriaproteinuria and saliva were 92% and 105%,respectively,with a relative standard deviation of 1.8%（n=6）.The common materials in organism had basically no interforrence.Conclusion The method can be applied to the determination of tota1 amount of protein in biological samples with high sensitivity,better stability and anti-interference.

关 键 词：蛋白质 镧(Ⅲ)-铬天青S配合物探针 分光光度法 

分 类 号：Q591.2[生物学—生物化学]