正己烷对大鼠卵巢颗粒细胞凋亡调控基因影响  

Effects of n-hexane on expression of apoptosis regulator genes in rat ovarian granulosa cell

作　　者：黄磊[1] 欧阳江[1] 刘瑾[1] 林炜[1] 黄慧玲[2] 张文昌[1] 

机构地区：[1]福建医科大学公共卫生学院职业与环境卫生学系环境与健康重点实验室,福建福州350004 [2]福建医科大学附属协和医院

出　　处：《中国公共卫生》2011年第3期338-339,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(30972514)

摘　　要：目的观察正己烷对大鼠卵巢颗粒细胞凋亡调控基因bcl-2、bax、bcl-xl、xiap mRNA表达的影响。方法 48只成年雌性Wistar大鼠按体重随机分为4组,分别给予浓度为0,3.01,9.03,27.1 g/m3正己烷静式吸入染毒;每天4 h,每周6 d,持续6周;染毒结束待大鼠进入动情期,处死并提取卵巢颗粒细胞,并采用实时荧光定量PCR法测定颗粒细胞bcl-2、bax、bcl-xl、xiap mRNA表达水平。结果随着染毒剂量增加,各组bcl-2 mRNA表达呈下降趋势(2-△△CT值分别为1.069,0.762,0.698,0.690),bax mRNA表达呈上升趋势(2-△△CT值分别为1.039,1.242,1.317,1.895),各染毒组与对照组比较,差异均有统计学意义(P<0.05)。各染毒组bcl-xl mRNA表达与对照组差异无统计学意义(P>0.05);27.1g/m3染毒组xiap mRNA表达(0.694)低于对照组(0.999),差异有统计学意义(P<0.05)。结论正己烷上调大鼠卵巢颗粒细胞bax,下调bcl-2、xiap mRNA表达,可能是正己烷致使卵巢颗粒细胞凋亡的机制之一。Objective To observe the effects of n-hexane on the expression of apoptosis regulator gene bcl-2,bax,bcl-xl,and xiap mRNA in rat ovarian granulosa cells.Methods Totally 48 adult female rats were randomly assigned to four groups based on weight and were exposed to 0,3.01,9.03,and 27.1 g/m3 n-hexane for 6 weeks（4h/d,6 days/week）,respectively.After n-hexane inhalation,the rats in estrus stage were killed and their ovarian granulosa cells were collected.The expressions of bcl-2、bax,bcl-xl,and xiap mRNA were measured with real-time fluorescence quantitative PCR method.Results With the increased n-hexane concentration,the expression of bcl-2 mRNA decreased（the value of 2-△△CTwas 1.069,0.762,0.698,0.690,respectively）and the expression of bax mRNA increased gradually（the value of 2-△△CTwas 1.039,1.242,1.317,1.895,respectively）,both of them in n-hexane groups showed significant difference compared with those of in the control group（P0.05）.The bcl-xl mRNA expression in all n-hexane groups showed no significant difference compared with that of the control group（P0.05）.The expression of xiap mRNA in 27.1g/m3 group（the value of 2-△△CTwas 0.694） decreased significantly compared with that of in the control group（the value of 2-△△CTwas 0.999,P0.05）.Conclusion N-hexane could enhance bax mRNA expression and downregulate bcl-2 and xiap mRNA expression,which might be the mechanism of apoptosis of rat ovarian granulosa cell.

关 键 词：正己烷 颗粒细胞 bcl-2 bax BCL-X1 XIAP 

分 类 号：R135.1[医药卫生—劳动卫生]